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先天性巨结肠患儿结肠肌间神经丛内神经节细胞中NKX2-1的表达 被引量：1

Expression of transcription factor NKX2-1 in the colon of children with Hirschsprung's disease
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摘要 目的:探讨转录因子NKX2-1在先天性巨结肠(HD)患儿结肠肌间神经丛内神经节细胞中的表达。方法:应用免疫组化SP和RT-PCR技术检测30例HD患儿狭窄、移行和扩张段结肠组织和30例正常对照结肠组织中NKX2-1的表达情况。结果:HD患儿狭窄、移行段结肠组织中NKX2-1蛋白和mRNA的表达均低于正常对照组,差异有统计学意义(Z=5.236和2.926,P<0.008;t/t’=19.475和14.429,P<0.001)。结论:转录因子NKX2-1在结肠肌间神经丛内神经节细胞中表达减少可能与HD的发生有关。 Aim：To investigate the expression of transcription factor NKX2-1 and its significance in the colon of children with Hirschsprung＇s disease（HD）.Methods：The protein and mRNA expressions of NKX2-1 in narrow,transition,and expansion segments of colon tissues from 30 cases of HD and 30 normal control were examined by immunohistochemistry and RT-PCR.Results：The expressions of NKX2-1 protein and mRNA in narrow and transition segments from colon tissue of HD were lower than those of normal colon tissue（protein：Z=5.236 and 2.926,P0.008;mRNA：t/t＇=19.475 and 14.429,P0.05）.Conclusion：The reduction of NKX2-1 in the wall of colon may participate in the pathogenesis of HD.
作者 陈琦 王志 曹振杰 赵金磊 冯伟楼
机构地区 郑州大学第三附属医院小儿外科
出处 《郑州大学学报（医学版）》 CAS 北大核心 2012年第3期365-367,共3页 Journal of Zhengzhou University(Medical Sciences)
关键词 先天性巨结肠 NKX2-1 儿童 Hirschsprung＇s disease NKX2-1 children
分类号 R726.1 [医药卫生—儿科]
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共引文献13

同被引文献8

  • 1杨合英,刘秋亮,王家祥,许华峰.多发性节段型肠无神经节细胞症的临床分析[J].中华医学杂志,2005,85(39):2772-2774. 被引量：6
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  • 8彭梓月,韩毅敏,王晶.L1-CAM在肿瘤组织中的研究进展[J].现代肿瘤医学,2013,21(3):665-668. 被引量：2

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郑州大学学报（医学版）
2012年 第3期

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