白香丹胶囊对愤怒情绪模型大鼠海马GABABR和KIR表达的影响

Effect of Baixiangdan Capsule on Expression of GABABR and KIR in Hippocampus of Anger-out Rat Models

目的检测愤怒情绪模型大鼠海马γ-氨基丁酸B受体两个亚基GABABR1、GABABR2和内向整流型钾通道(KIR)表达的变化,初步探讨白香丹胶囊对肝气逆证的中枢干预机制。方法大鼠随机分为正常对照组、模型组、白香丹胶囊组(0.2 g·kg-1)、巴氯芬组(0.008 g·kg-1),采用"社会隔离结合居住入侵"方法制备愤怒情绪大鼠模型,药物组按相应剂量灌胃给药7 d,每日1次。通过糖水偏好实验、旷场实验和高架十字迷宫实验进行模型评价,用免疫荧光化学法检测各组大鼠海马GABABR1、GABABR2和KIR的表达。结果与正常对照组比较,模型组糖水偏好系数降低,旷场实验得分增高,高架十字迷宫实验进入开放臂次数百分数(OE%)和开放臂停留时间的百分数(OT%)降低,海马GABABR1、GABABR2和KIR表达水平降低P<0.05;与模型组比较,白香丹胶囊组和巴氯芬组旷场实验得分降低,OE%和OT%值升高,海马GABABR1、GABABR2和KIR表达水平升高(P<0.05)。结论大鼠愤怒情绪的产生可能与海马GABABR1、GABABR2和KIR表达降低有关,白香丹胶囊平肝理气的中枢机制可能与其激活海马GABABR及其G蛋白偶联的KIR有关。

Objective To detect the expression of two subunits of gamma aminobutyric acid B receptor, GABABR1 andGABABR2, as well as inwardly rectifying K＋ channel（KIR） in hippocampus of anger-out rat models, and to ex- plore the intervention mechanism of Baixiangdan Capsule in teating the syndrome of adverse flow of liver-Qi. Methods The Wistar rats were divided into normal group, modle group, Baixiangdan Capsule group（ig 0.2 g·kg^-1, qd, for 7 d） and baelofen group （ig 0.008 g·kg^-1, qd, for 7 d）. The anger-out emotion rat models were established with social isolation plus resident intruder stress. The model was evaluated by sucrose intake test, open field test and elevated plus-maze test. The expression of GABABR1, GABABR2 and KIR in hippocampus was detected by immunofloures- cence assay. Results Compared with the normal group, the modle group had lower sucrose intake, higher total score of open field test, lower open arm entry percent（OE %） and open arm time percent（OT %） of elevated plus-maze test, and lower expression of GABABRI, GABABR2 and KIR in hippocampus（P 〈 0.05）. Compared with the model group, Baixiangdan capsule and baclofen decreased the total score of open field test, increased OE % and OT % of elevated plus-maze test, and promoted expression of GABABR1, GABABR2 and KIR in hippocampus（P 〈 0.05）. Conclusion The lower expression of GABABR1, GABABR2 and KIR in hippocampus may have close relationship with anger-out emotion. Increasing the expression of GABABR and the function of G protein-coupled KIR in hip- pocampus maybe one of the mechanism of Baixiangdan capsule in soothing liver and regulating Qi flow.