正交实验优化金银花中绿原酸超声提取工艺

Orthogonal Test Design for Optimization of Ultrasonic Extraction Process of Chlorogenic Acid from Honeysuckle

目的:优化金银花中绿原酸超声提取工艺。方法:应用正交实验设计优化绿原酸的超声提取工艺,采用高效液相色谱法测定绿原酸含量。结果:影响超声提取绿原酸的因素为溶剂类型>预处理>提取时间>物料比。最佳超声提取工艺是60%乙醇为溶剂,物料比1:30,提取时间45min,远红外微波1min。结论:超声微波应用于金银花中绿原酸的提取、具有提取率高、省时、高效、节能等优点。

Objective：To optimize the ultrasonic extraction process of chlorogenic acid from honeysuckle.Methods：Design orthogonal test to optimize the ultrasonic extraction process of chlorogenic acid,and measure the amount of chlorogenic acid by High Performance liquid chromatography（HPLC）.Results：The order of factors that affect the chlorogenic acid extraction process was solvent type 〉 pretreatment 〉 extraction time 〉 material ratio.In optimal ultrasonic extraction process,60% ethanol is solvent,material ratio is 1：30,and extraction time is 45min,with far infrared microwave 1min.Conclusion：Application of ultrasonic microwave to the chlorogenic acid extraction process results higher extraction rate,saves time and energy,which is more efficient.