产共轭亚油酸鼠李糖乳杆菌的筛选与鉴定

Screening and Identification of Conjugated Linoleic Acid-producing Lactobacillus rhamnosus

从东北传统酸菜汁中筛选出具有产共轭亚油酸(CLA)能力的乳酸菌菌株15株,采用紫外分光度计法测定发酵液中共轭亚油酸产量,其中菌株A53.2的产量最高,在MRS培养基中添加0.4mg/mL亚油酸(LA),37℃发酵24h,CLA产量达14.91μg/mL。通过对菌体的形态特征和生化特性分析,并结合16SrDNA分子生物学以及系统发育分析鉴定菌株A53.2为鼠李糖乳杆菌。

This study was to screen out 15 lactic acid bacteria strains from the northeast traditional fermented sour pickled cabbage and these strains have the ability to product conjugated linoleic acid （CLA）. The CLA production was determinated by ultraviolet spectrophotometry. Strain A53.2 had the highest capacity to produce CLA, and by being cultured in MRS medium with 0.4mg/mL linoleic acid （LA） for 24 h at 37℃, the yield of CLA in fermentation broth was 14.91μg/mL. Combining with 16SrDNA gene sequence analysis and phylogenetic analysis, the strain A53.2 was identified to be Lactobacillus rhamnosus by analyzing the configuration, cell characteristics, and biochemical characteristics.