摘要
目的:探讨白藜芦醇对紫外线照射后人皮肤角质形成细胞水通道蛋白3(AQP3)表达的影响及意义。方法:原代培养人皮肤角质形成细胞,采用UVB(20mJ/cm2,40mJ/cm2)照射角质形成细胞后,立即加入0.1mmol/L的白藜芦醇进行干预。RT-PCR检测照射前后角质形成细胞中AQP3 mRNA的表达量,并用羟胺法、比色法、TBA法检测照射前后细胞超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)含量。结果:1.UVB照射后角质形成细胞AQP3 mRNA的表达量下降(P<0.05),且UVB照射剂量越大,AQP3 mRNA下降越显著(P<0.05)。2.白藜芦醇能显著增加UVB照射后角质形成细胞SOD和GSH-Px活性,并降低细胞MDA含量(P<0.05)。3.白藜芦醇能显著抑制UVB导致的角质形成细胞AQP3 mRNA下降(P<0.05)。结论:白藜芦醇可能通过抑制UVB导致的AQP3 mRNA下降,及提高氧化酶活性、清除自由基的功能,从而延缓皮肤衰老。
Objective： To investigate the effect of resveratrol on expression of AQP3 in normal human epidermal keratinocytes （NHEK） irradiated by UVB, and discuss its function in the process of skin aging. Methods： Preparation of skin keratinocyte primary cultures. NHEK irradiated by UVB （20mJ/cm^2, 40mJ/m^2） were immediately treated with 0. 1mmol/L resvetatrol, then observe the change of the expression ofAQP3 mRNA by RT-PCR, and the change of SOD, GSH-Px and MDA by Hydroxyl amine method, colorimetric me- thod and TBA method. Results： 1. The expression level ofAQP3 mRNA after UVB irradiation was significantly lower than its level bef- ore UVB irradiation （P〈0.05）. The more the UVB exposure dose it received, the greater the expression level ofAQP3 mRNA decreased. 2. Resvetatrol increased the activity of SOD and GSH-Px, decreased MDA＇s content of NHEK irradiated by UVB （P〈0.05）. 3. Resvetatrol inhibited the decrease ofAQP3 mRNA irradiated by UVB （P〈0.05）. Conclusion： Resvetatrol can attenuate UVB-induced down-regulation of AQP3, increase oxydase＇s activity and clean the free radical, and accordingly to delay skin aging.
出处
《现代生物医学进展》
CAS
2012年第12期2275-2277,2293,共4页
Progress in Modern Biomedicine
关键词
白藜芦醇
水通道蛋白3
角质形成细胞
中波紫外线
Resvetatrol
Aquaporin 3（AQP3）
Normal human epidermal keratinocytes（NHEK）
Ultraviolet B（UVB）
