摘要
目的:对2010年北京市某两区发生的儿童甲3亚型流行性感冒暴发疫情进行病原学研究。方法:荧光RT-PCR方法检测疫情患者标本,对核酸阳性者进行病毒培养;对毒株血凝素HA1基因进行序列比对和进化分析。结果:两起疫情标本甲3亚型流感病毒核酸阳性检出共14件;毒株共分离到6株。各疫情毒株同源性序列为100%;两起疫情的HA1序列同源性为97.3%。与疫苗株A/Perth/16/2009(H3N2)比较,其中疫情2分离株共有8个氨基酸(aa)变异。结论:两起疫情均为甲3亚型(H3N2)流感所致;同一起疫情均由同一病毒株传播所致;两起疫情甲3亚型(H3N2)毒株的血凝素HA1区存在不同程度的aa变异。
Objective：To conduct the etiological analysis of two outbreaks of H3N2 influenza in different areas of Beijing.Methods： Nasopharyngeal swab specimens were collected and tested for influenza virus by real time RT-PCR.Positive specimens were isolated for cell culture.Furthermore,hemagglutin subunit（HA1） genes were amplified,sequenced and aligned with A/Perth/16/2009（H3N2）.Results： The etiology of these two outbreaks was H3N2 virus,with a total of fourteen positive specimens by real time RT-PCR.In addition,a total of six virus strains were isolated.HA1 sequences analysis showed that every outbreak was caused by the same virus strain.Compared with the sequence of A/Perth/16/2009（H3N2）,the virus strains from outbreak 2 had eight amino acid mutations.Conclusion： These two outbreaks were caused by H3N2 virus.Furthermore,amino acid mutations were found in HA1 region of the virus strains with different degrees.
出处
《中国卫生检验杂志》
北大核心
2012年第5期968-970,973,共4页
Chinese Journal of Health Laboratory Technology
