流式细胞术在AA和L-MDS鉴别诊断中的临床价值

Application of flow cytometry in differential diagnosis of AA and L-MDS

目的:探讨流式细胞术在再生障碍性贫血(AA)和低增生型骨髓增生异常综合征(L-MDS)鉴别诊断中的临床价值。方法:采用多色流式细胞术检测5例L-MDS和7例AA患者骨髓粒细胞免疫表型,并对其血象和免疫表型进行比较。结果:L-MDS和AA患者外周血血红蛋白、造血岛个数、粒红淋系比例差异均存在显著统计学意义(P<0.01,P<0.05)。L-MDS和AA患者骨髓CD34+、CD117+细胞比例、粒系CD7表达、CD15缺失、CD45平均荧光强度(MFI)异常、CD13/CD16图形异常比例差异存在显著统计学意义(P<0.01,P<0.05)。结论:流式检测骨髓病态造血有助于L-MDS和AA的鉴别诊断。

Objective：To investigate the application value of flow cytometry（FCM） in differential diagnosis of low-proliferation myelodysplastic syndromes（L-MDS） and aplastic anemia（AA）.Methods： The immunophenotype of bone marrow（BM） in 5 patients with L-MDS and 7 patients with AA were detected by FCM.Their clinical datas and immunophenotypes were compared.Results： The hemoglobin,islands,ratios of granulocyte,nucleated erythrocyte,monocyte in peripheral blood were significantly different between patients with L-MDS and AA（P〈0.01 or P〈0.05）.The ratios of CD34＋ cells,CD117＋ cells,granulocyte CD7 expression,granulocyte CD15 loss,abnormal granulocyte CD45 mean fluorescence intensity（MFI）,abnormal granulocyte CD13/CD16 graph in BM were significantly different between patients with L-MDS and AA（P〈0.01 or P〈0.05）.Conclusion： Bone marrow（BM） dyshaematopoiesis detection by FCM is of great value for differential diagnosis of L-MDS and AA.