RP-HPLC法测定浓维磷糖浆中咖啡因和维生素B_1的含量

RP-HPLC determination of the content of caffeine and vitamine B1 in concentrated divitamins and sodium phosphate syrup

目的:采用反相高效液相色谱法测定浓维磷糖浆中咖啡因和维生素B1的含量。方法:采用Dia-monsil C18柱(250 mm×4.6 mm,5μm),以甲醇-庚烷磺酸钠缓冲液为流动相(10∶90),检测波长为272 nm。结果:咖啡因与维生素B1浓度分别在3.5μg/ml～282.6μg/ml(r=1.0000)和0.7μg/ml～54.7μg/ml(r=0.9999)内与峰面积呈良好的线性关系(n=9),方法回收率(n=9)分别为99.2%和99.1%,RSD分别为1.8%和1.7%。结论:该方法操作简便,结果准确可靠,可以有效地控制产品质量。

Objective：To establish a method for determination of caffeine and vitamine B1 in concentrated divitamins and sodium phosphate syrup.Methods： The reversed phase HPLC system consisted of a C18 column（250 mm＊4.6 mm,5 μm）.The mobile phase consisted of methanol and heptanesulfonate sodium（10∶ 90）,and the wavelength of detector was 272 nm.Results： The linear response range of caffeine and vitamine B1 were 3.5 μg/ml^282.6 μg/ml（r=1.0000） and 0.7 μg/ml^54.7 μg/ml（r=0.9999）,respectively（n=9）.The average recovery rates（n=9） of caffeine and vitamine B1 were 99.2%（RSD=1.8%） and 99.1%（RSD=1.7%）,respectively.Conclusion： This method is simple and convenient in work.It is accurate and suitable for the quality control.