水产品中霍乱弧菌的监测方法研究

Study on the monitoring methods of Vibrio cholerae in aquatic products

目的:研究水产品中霍乱弧菌的监测方法,及时发现被霍乱弧菌污染的水产品,采取有效防控措施,防范霍乱疫情的发生。方法:用实时荧光PCR法、胶体金法、分离培养法三种方法分别对180份水产品进行霍乱弧菌检测,阳性样品再进行霍乱肠毒素检测。结果:三种方法检出了44份核酸阳性标本,18份霍乱肠毒素阳性标本,分离出8株霍乱弧菌。结论:在进行水产品霍乱监测时,可以先用实时荧光PCR进行初筛,筛出核酸阳性标本,进行霍乱肠毒素检测,及时发现被霍乱产毒株污染的水产品,再对筛出的核酸阳性标本结合胶体金法进行分离培养和分型鉴定,进一步完成霍乱疫情的确证和分析。

Objective：To study the monitoring methods of Vibrio cholerae in aquatic products,find the aquatic products contaminated by Vibrio cholerae timely,and take effective measures to prevent and control cholera outbreak.Methods： The real-time fluorescence PCR,the colloidal gold method,the isolation and culture method were used for Vibrio cholerae detection in 180 aquatic product samples respectively.Results： With the three methods,44 nucleic acid positive specimens were detected,in which 18 specimens were cholera enterotoxin positive,and 8 Vibrio cholerae strains were isolated.Conclusion： During cholera surveillance of aquatic products,nucleic acid positive samples were screened first by real-time PCR,and then cholera enterotoxin detection was done to find the polluted aquatic products timely.Colloidal gold method was employed to isolate,culture and type the nucleic acid positive samples for further confirmation and analysis of cholera spread.