PTEN异常表达及甲基化与膀胱移行细胞癌的关系

Relationship between mRNA expression and promoter methylation of PTEN gene in transitional cell carcinoma of bladder

目的:探讨PTEN基因mRNA异常表达及其启动子甲基化与膀胱移行细胞癌的关系。方法:应用逆转录聚合酶链反应(RT-PCR)技术检测膀胱移行细胞癌组织48例和正常对照15例PTEN基因mRNA的表达水平和应用甲基化特异性PCR(MSP)法检测启动子的甲基化。结果:PTEN基因mRNA在膀胱癌患者的表达在48例中有27例,而正常对照全部有表达;正常对照全部未检测到PTEN基因启动子甲基化,而膀胱癌患者启动子甲基化有23例。结论:PTEN基因mRNA表达缺失可能与启动子甲基化有关,在膀胱移行细胞癌的发生、发展及转移过程中起重要作用。

Objective：To probe the significance of mRNA aberrant expression and promoter methylation of PTEN gene in clinical pathological features of transitional cell carcinoma of bladder（TCCB）.Methods： A total of 48 patients with TCCB and 15 normal controls were detected for mRNA expression and promoter methylation of PTEN gene by reverse transcription polymerase chain reaction（RT-PCR） and methylation specific PCR（MSP） techniques respectively.Results： The PTEN mRNA positive expression in TCCB was 27 in 48 cases,but all positive in normal controls.23 in 48 TCCB cases had methylation of promoter,but none in normal controls.Conclusion： The loss of mRNA expression of PTEN gene is relevant with the aberrant methylation of its promoter,and it might be one of the reasons for the generation,development and metastasis of TCCB.