茜素紫共振瑞利散射光谱法测定人唾液中溶菌酶含量

Determination of lysozyme in human saliva with Alizarin violet by resonance rayleigh scattering

目的:建立一种以茜素紫为探针共振瑞利散射技术测定溶菌酶的方法。方法:唾液样品处理后,在优化测定条件下以茜素紫为探针共振瑞利散射技术测定其中的溶菌酶。结果:溶菌酶的检出限为0.43 mg/L(3S/N);溶菌酶标准溶液在2.0 mg/L～30.0 mg/L范围内线性良好,其相关系数分别为0.999;测定8.0 mg/L溶菌酶标准溶液的相对标准偏差(RSD)3.0%。样品加标回收率:97.2%～104.4%。结论:该方法灵敏度、准确度高;操作方便快速;具有较低的检出限,能满足测定人唾液中溶菌酶含量的检测工作。

Objective：To establish a method for determination of lysozyme in saliva by resonance rayleigh scattering（RRS） probe.Methods： Under the proposed conditions,lysozyme was determined in saliva by RRS probe.Results： The detection limits were 0.43 mg/L（3S/N） for lysozyme.The liner calibration curve for lysozyme was in the range of 2.0 mg/L^30.0 mg/L,and their correlative coefficient was 0.999.The RSD for lysozyme at 8.0 mg/L was 3.0%.The standard recovery rates of spiked samples were in the range of 97.2%~104.4% for lysozyme.Conclusion： The method is simple,rapid,accurate,sensitive with low detection limits,which is suitable for the determination of lysozyme in saliva.