实时荧光PCR检测EV71病毒核酸的临床应用价值

Clinical application of real time fluorescence PCR in EV71 virus nucleic acid detection

目的:讨论实时荧光PCR技术检测EV71病毒核酸的临床价值。方法:对128例临床诊断为手足口病患者采用实时荧光RT-PCR技术检测患者粪便中EV71病毒核酸,分析结果与临床诊断相符性。结果:128例临床诊断为手足口病的患儿经实时荧光RT-PCR检测EV71病毒核酸阳性者65例(50.78%)。结论:实时荧光RT-PCR技术是诊断手足口病的主要实验方法,但该方法存在一定的漏检,临床诊断手足口病时应密切结合临床以防漏诊。

Objective：To explore the value of real time fluorescence PCR in detection of EV71 virus.Methods： A total of 128 HFMD patients were tested by real time fluorescence PCR for the EV71 viral nucleic acid,the consistency of Real time fluorescence RT-PCR results and the clinical diagnosis was analyzed.Results： EV71 virus were positive in 65 of 128（50.78%） patients for clinical diagnosis of HFMD with real time fluorescence RT-PCR.Conclusion： Real-time fluorescene RT-PCR was the main experimental method for diagnosis of HFMD,which had a certain disadvantages,so missed diagnosis should be paid more attention in diagnosis of HFMD.