妊娠期糖尿病患者胎盘和腹部皮下脂肪组织中脂联素受体2的表达分析

Expression of adioponectin receptor 2 in abdominal subcutaneous adipose tissues and placental tissues of patients with gestational diabetes mellitus

目的探讨脂联素受体2(adioponectin receptor 2,AdipoR 2)在妊娠期糖尿病患者胎盘及腹部皮下脂肪组织中的表达情况。方法收集正常妊娠妇女及妊娠期糖尿病患者各20例皮下脂肪组织及胎盘组织,分别采用逆转录聚合酶链反应技术(RT-PCR)及蛋白免疫印迹法(Western bolt)检测AdipoR 2 mRNA及其蛋白质的表达情况。结果与正常妊娠妇女相比较,妊娠期糖尿病患者胎盘组织中AdipoR 2 mRNA及其蛋白质表达均升高,皮下脂肪组织中Adi-poR 2 mRNA及其蛋白质表达均降低。结论 AdipoR 2在胎盘组织及皮下脂肪组织中表达异常,可能在妊娠期糖尿病的发病机制中起到重要作用。

Objective To investigate the expression of adioponectin receptor 2（AdipoR 2） in abdominal subcutaneous adipose tissues and placental tissues of the patients with gestational diabetes mellitus（GDM）.Methods The expressions of AdipoR2 mRNA and its protein in abdominal subcutaneous adipose tissues and placental tissues in 20 normal pregnant women（control group） and 20 patients with gestational diabetes mellitus（GDM group） were measured by RT-PCR and Western blot.Results Compared with the control group,the levels of AdipoR2 mRNA and its protein in placental tissues in the GDM group were significantly higher.While the levels of AdipoR2 mRNA and its protein in abdominal subcutaneous adipose tissues in GDM group were significantly reduced.Conclusion AdipoR2 expression is up-regulated in placenta tissues but down-regulated in abdominal subcutaneous adipose tissue,which plays an important role in mediating the effects of adiponectin in GDM.