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嗜碱菌与非嗜碱突变株膜蛋白比较 被引量:4

The Comparation of Membrane Proteins from Alkalopilic Bacteria and It's Mutant Non alkalopilic Strain
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摘要 分别提取不同条件下培养的兼性嗜碱菌NTT3 6及该菌株的非嗜碱突变株NTT3 6cs- 8(Eryr)的膜蛋白进行SDS -聚丙烯酰胺线性梯度凝胶电泳 ,发现膜蛋白质带有明显的不同 .推测这与嗜碱菌的嗜碱性有关 ,对嗜碱细菌的嗜碱机理作一初探 。 The membrane proteins of NTT 36 and NTT 36 cs-8(Ery r) were extracted and run gradient SDS PAGE.The obvious difference of membrane proteins in these bacteria by gel electrophoresis was found.The difference may result from the alkalophilia of alkalophilic bacteria.It is necessary to understand the alkalophilic mechanism and to promote the utilization of alkalophilic bacteria.
机构地区 云南大学生物系
出处 《云南大学学报(自然科学版)》 CAS CSCD 2000年第2期148-149,153,共3页 Journal of Yunnan University(Natural Sciences Edition)
关键词 兼性嗜碱菌 膜蛋白 凝胶电泳 非嗜碱突变株 facultative alkalophiles membrane protein gradient SDS PAGE
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  • 1陈永青,宋大新,许钢锋.嗜碱性芽孢杆菌启动子的克隆及某些因子对表达的影响[J]微生物学报,1987(04).
  • 2Terry Ann Krulwich,Arthur A. Guffanti. The Na+ cycle of extreme alkalophiles: A secondary Na+/H+ antiporter and Na+/solute symporters[J] 1989,Journal of Bioenergetics and Biomembranes(6):663~677
  • 3Hideto Takami,Teruhiko Akiba,Koki Horikoshi. Production of extremely thermostable alkaline protease from Bacillus sp. no. AH-101[J] 1989,Applied Microbiology and Biotechnology(2):120~124
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  • 6黄诗笺,卢振祖,陈漱涢.嗜碱细菌碱性果胶酶的研究——Ⅰ.菌种筛选、产酶条件和酶作用条件的研究[J].武汉大学学报(自然科学版),1990(1):105-110. 被引量:5

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