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Survivin基因与Bcl-2/bax在不同肝癌细胞中的表达研究

Survivin and Bcl-2 /bax Expression Human in Hepatoma SMMC-7721 and Hep-G2 Cells as well as Theirs relative
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摘要 目的:探讨Survivin基因在肝癌细胞中的表达及与Bcl-2/bax的关系。方法:体外培养的人肝癌细胞SMMC-7721、Hep-G2及正常肝细胞,采用RT-PCR法,免疫组织化学检测Survivin及Bcl-2/bax基因的表达情况。结果:免疫组织化学法显示肝癌细胞中有Survivin基因表达,且在SMMC-7721比在Hep-G2表达更明显,而在正常肝细胞中Survivin基因表达不明显;Survivin高表达的细胞,Bcl-2也增高。RT-PCR法显示Survivin基因的表达在肝癌细胞中增加。结论:Survivin基因与人肝癌细胞SMMC-7721及Hep-G2有关,与肝癌细胞的分型相关,由此推断,Survivin基因与肝癌的发生、发展及预后有关。 Objective： This study was aimed at investigating surviving and bcl- 2/bax expression in human hepatoma cell SMMC- 7721and Hep- G2 line as well as theirs relative. Methods： Immuno- histological assay were applied to detect the expression of surviving and Bel - 2 /bax genes in the hepatoma SMMC- 7721 and Hep - G2 cells. Survivin and bcl - 2/bax protein and mRNA expressions were measured with reverse transcrip- tion polymerase chain reaction （RT- PCR） , respectively. Results： RT- PCR analyses showed survivin and bcl - 2/bax expression in SMMC - 7721 cell increasing and in the Hep - G2 cell posteriorly but no expression in the natural liver. Immuno - histological assay revealed the same results with the RT - PCR. Conclusions： Sur- vivin gene is related with human hepatoma cell SMMC - 7721 and Hep - G2. Suvivin gene may play a role in in- hibiting apoptosis in liver cancer and may function importantly in tumor production and development.
作者 宝金才 刘俊彪 刘彦恒 李军华
机构地区 内蒙古鄂托克旗人民医院外科 内蒙古自治区人民医院肿瘤外科
出处 《内蒙古医学杂志》 2012年第4期397-399,共3页 Inner Mongolia Medical Journal
关键词 SURVIVIN BCL-2/BAX 肝癌细胞 Survivin Bcl - 2/bax Carcinoma hepat ocellular
分类号 R734 [医药卫生—肿瘤]
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参考文献10

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二级参考文献11

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内蒙古医学杂志
2012年 第4期

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