不同超排方案以及重复超排对犊牛超排效果的影响

Effect of Different Superovulation Schemes and Repeated Superovulation on Calf Superovulation

本试验选择北京奶牛中心良种场36头9～12月龄的犊牛进行超排采卵，研究了不同超排方案以及重复超排对犊牛超排的效果。结果表明，超排方案以埋植CIDR的同时，第5天、第6天注射FSH，第7天手术采卵获得的可用卵母细胞数最多。两次重复超排对犊牛平均卵泡数和平均卵母细胞数没有显著影响，但三次超排后的平均卵泡数和平均卵母细胞数显著低于一次和二次超排。

In this study, 9-12 week-old calves were superovulated by hormone, the results showed that the number of follicles and oocytes were different with different superovulation schemes, the best method is implant CIDR on l d, injection of FSH on 5d and 6d, surgical collection oocytes on 7d, can obtain more follicles and oocytes. The calves were repeated superovulated for second and third times, results showed that the average number of follicles and oocytes were no significant difference between superovulation and second times superovulation, but the average number of follicles and oocytes were significantly lower than the first and second superovulation.