狂犬病毒融合基因pVax-G/N的克隆

Cloning of rabies virus fusion gene pVax-G/N

目的构建狂犬病病毒G基因和N基因的融合表达载体,为研究融合基因疫苗打下基础。方法利用分子生物学技术从质粒pVax-G中克隆出狂犬病毒G和N基因,经连接及鉴定成功后与酿酒酵母表达载体pYes2连接,并进行酶切及测序鉴定。结果融合基因pVax-G/N测序结果与预期完全符合。结论成功构建了融合表达载体pYes2-pVax-G/N,为进一步研究稳定、安全的狂犬病疫苗奠定基础。

Object To construct rabies virus gene expression fusion vector of N and G genes for laying the foundation of the study on fusion gene vaccine.Methods Molecular biology technology was being employed to clone hydrophobic poison G and N gene from the plasmid pVax-G.After the success of the connection and appraisal,the cloned G and N gene were connected with wine yeast expression vector pYes2.And then the recombinant plasmid of G and N gene were appraised by restriction enzyme digestion and sequencing.Results The sequencing result of fusion gene pVax-G/N was consistent with excepted results.Conclusion Fusion expression vector of pYes2-pVax-G/N were successfully constructed,which would lay the foundation to study further on the stability and safety of rabies vaccine.