西花蓟马诱导的番茄植株防御反应对B型烟粉虱行为的影响

Effects of defense responses in tomato plants induced by Frankliniella occidentalis(Thysanoptera:Thripidae) on behavioral responses of Bemisia tabaci B-biotype(Hemiptera:Aleyrodidae)

【目的】明确外来入侵害虫西花蓟马Frankliniella occidentalis(Pergande)取食诱导的番茄植株防御反应对B型烟粉虱Bemisia tabaci(Gennadius)B-biotype行为的影响,探讨西花蓟马与B型烟粉虱的种间竞争机制。【方法】利用"Y"型嗅觉仪行为分析法、选择性试验法,研究了不同处理组合(健康植株CKvs烟粉虱为害植株B、健康植株CKvs西花蓟马为害植株F、烟粉虱为害植株Bvs烟粉虱和西花蓟马共同为害植株B+F)下,烟粉虱对不同处理植株的取食行为反应及寄主偏好的差异性,并利用GC-MS分析了不同处理植株挥发性物质的差异性。【结果】"Y"型嗅觉仪行为分析结果表明,在CKvsB和CKvsF两个组合中,烟粉虱雌成虫对健康植株和昆虫为害植株的偏好性差异不显著;而在BvsB+F组合中,烟粉虱为害株对烟粉虱雌成虫具有极显著的引诱作用(P<0.01)。选择性试验结果表明,在CKvsB和CKvsF这两个组合中,烟粉虱成虫均偏好健康株并喜好在其上产卵(P<0.01),在BvsB+F组合中,烟粉虱成虫偏好烟粉虱单独为害株并喜好在其上产卵(P<0.01)。GC-MS分析结果显示,本实验收集到9种挥发性物质,其中有5种烯萜类物质,其相对含量高达90%以上。与烟粉虱单独为害株相比,烟粉虱和西花蓟马共同为害株所释放的挥发性物质中,对烟粉虱具有排斥作用的β-水芹烯显著升高,而对其具有吸引作用的β-月桂烯却显著减少。【结论】综合分析认为,西花蓟马诱导的番茄植株防御反应可以显著影响B型烟粉虱的寄主选择行为及产卵选择性。

[ Aim ] To understand the effects of defense responses in tomato plants induced by Frankliniella occidentalis （Pergande） on behavioral responses of Bemisia tabaci （Gennadius） B-biotype, and to explore the interspecific competition mechanisms between F. occidentalis and B. tabaci B-biotype. [ Methods] Using Y-tube olfactometer and choice test methods, we studied the differences of host plants damaged by F. occidentalis （F） and the plants damaged by B. tabaci plants （B） vs the plants damaged by B. tabaci and F. occidentalis （B ＋ F）, and identified the volatiles of tomato plants in different treatments by GC-MS. [ Results ] The olfactory responses of female B. tabaci adults were not significantly different between CK vs B and CK vs F treatment combinations, whereas strong selection reaction of female adults of B. tabaci to tomato plants damaged only by B. tabaci was found in B vs B ＋ F treatment combination （P 〈0.01 ）. The results of choice test showed that B. tabaci adults were both strongly attracted by the undamaged plants and oviposited on them in CK vs B and CK vs F treatment combinations （P 〈 0.01 ）, whereas B. tabaci adults prefered the plants damaged only by B. tabaci and oviposited on them in B vs B ＋ F treatment combination （P 〈 0.01 ）. The results showed that 9 kinds of plant volatiles were collected, 5 of which were alkenes, accounting for 90% of the total volatiles by GC- MS. β-Myrcene, which shows strong attractiveness to B. tabaci, remarkably reduced and β- phellandrene, which shows rejection to B. tabaci, remarkably increased in the plants damaged by both herbivores, compared to the plants damaged only by B. tabaci. [ Conclusion] The defense responses in tomato plants induced by F. occidentalis have a great effect upon host preference and behavioral responses of B. tabaci.