土贝母水提物对体外培养人肝癌细胞增殖及代谢的影响

Effects of BPF extracts on proliferation and mitochondria metabolism of human hepatoma SMMC-7721 cells

目的 研究中药土贝母(BPF)水提物对体外培养的人肝癌细胞的抑癌活性。 方法 BPF经粉碎、浸提、超速离心、减压浓缩、冷冻干燥得BPF水提物,人肝癌SMMC-7721细胞经BPF提取物处理不同时间后以MTT法测其对人肝癌细胞线粒体代谢活性的影响,以细胞记数法观察了其对细胞增殖的影响。 结果 ①经200mg/L BPF提取物处理48h,人肝癌SMMC-7721细胞线粒体活性为对照组的31.3％,处理72h为21.4％;②以100mg/L BPF提取物处理7d中,人肝癌细胞数分别为0.57,0.316,0.29,0.285,0.57,1.287,1.2,对照FCS组分别为1.82,3.67,6.2,9.4,10.1,11,9,NON组分别为1.7,3.35,5.6,8.4,8.9,9.3,7.6,实验组人肝癌细胞数不仅低于对照组,而且低于处理前细胞数(P<0.01);③经BPF提取物处理24h后,即使更换为正常培养基,人肝癌细胞数在96h的恢复培养中基本无增加。 结论 中药BPF提取物能显著抑制体外培养的肝癌细胞增殖和降低线粒体代谢活性。

AIM To demonstrate the effects of the extracts from traditional Chinese medicine Bolbostemma paniculatum (Maxim) Franquet (BPF) on the proliferation and mitochondria metabolism of human hepatoma SMMC-7721 cells.METHODS The dried BPF was ground to powder, extracted in cold distilled water and centrifuged to discard the pellet and the BPF supernatant was concentrated and lyophilized. After the dose-dependent experiments, human hepatoma cells were treated with 200mg/L BPF extract for 48 h and mitochondria metabolism activity was detected by MTT method.RESULTS BPF extracts could significantly decrease the activity of mitochondria metabolism, the A570 absorption in BPF treatment group was only 31.3% of that in control group after 48 h treatment and 21.4% after 72 h treatment; after 24 h treatment with BPF, the cell numbers in experimental group were 0.57,0.316,0.29,0.285,0.57, 1.287 and 1.2,in control group were 1.82, 3.67, 6.2, 9.4, 10.1 and 11, 9 and in the NON group were 1.7, 3.35, 5.6, 8.4, 8.9, 9.3 and 7.6. The numbers were not only lower than that in control group but also than that before treatment and maintained lower than that before treatment in the whole treatment (7d) (P<0.01); the cell numbers did not significantly increase after the cells were treated for 24 h and then replaced by culture in normal media for 96 h.CONCLUSION BPF extracts could inhibit the proliferation and mitochondria metabolism activity of human hepatoma cells.