乙肝E抗原阳性产妇卵巢组织中HBV cccDNA阳性的相关因素分析

Analysis of the factors correlated to HBV cccDNA in human ovarian in pregnant women with hepatitis B E antigen positive

目的检测人卵巢组织中HBV cccDNA,寻找人卵细胞为HBV靶细胞的证据。方法使用RT-PCR法测定33例慢性乙肝剖宫产手术产妇的卵巢组织HBV DNA、HBV cccDNA含量;同时检测孕妇的肝功能、HBV标志物与HBV DNA定量。结果产妇卵巢组织的HBV cccDNA与血清HBV DNA定量存在明显正相关性。其正相关因素为外周血HBsAg和HBV DNA定量(r=0.335),负相关因素为孕妇谷丙转氨酶水平(ALT,r=-0.360)及谷草转氨酶水平(AST,r=-0.347)。结论卵巢组织是HBV的靶器官,并可在其中复制。卵巢组织HBV cccDNA的存在与外周血HBV DNA载量、卵巢组织的HBV DNA载量和ALT、AST水平密切相关。

Objective To detect the existence of HBV ccc DNA in human ovrian infected with hepatitis B and prove the probability of the ovary being a target for HBV. Methods HBV DNA and HBV cccDNA in the ovarian were quantified by real-time quantitative-PCR in 33 pregnant women with hepatitis B E antigen positive undergoing Caesarean section. The liver function, blood markers of HBV and HBV DNA quantification were measured and analyzed as well. Results There was a significant positive correlation between the serum HBV DNA and ovarian HBV cccDNA, in which the positive relevant factors were HBsAg and HBV DNA quantification in peripheral blood, the negative relevant factors were ALT（r=-0. 360） and AST（r=-0. 347）. Conclusion The ovary is a target organ for HBV, in which HBV can replicate and pass to the next generation. The existence of HBV cccDNA in ovarian tissues is closely correlated to HBV DNA,AST and ALT in perypheral blood and HBV DNA quantification in the ovary.