补肾壮筋汤含药血清对力学刺激诱导软骨细胞凋亡的影响

Effects of Bushen Zhuangjin Decoction Containing Serum on the Apoptosis of Chondrocytes Induced by Mechanics Stimulus

目的探讨补肾壮筋汤含药血清对力学刺激诱导软骨细胞凋亡的影响。方法提取兔补肾壮筋汤含药血清,将软骨细胞培养基分为普通培养基组、空白兔血清培养基组(空白培养基组)及补肾壮筋汤培养基组(含药培养基组),对各组软骨细胞进行四点弯曲机械应力加载诱导凋亡。收集软骨细胞,流式细胞仪测定软骨细胞的凋亡率,并测定相应培养基中的IL-1β和NO的含量。结果含药培养基组中软骨细胞的凋亡[(19.55±7.98)%]比普通培养基组[(39.32±13.45)%]及空白培养基组[(37.87±9.67)%]低,差异有统计学意义(P<0.05);含药培养基组IL-1β和NO含量也低于其他两组,差异有统计学意义(P<0.05)。结论补肾壮筋汤含药血清对应力加载诱导软骨细胞的凋亡具有保护作用。

Objective To study the effects of Bushen Zhuangjin Decoction （BZD） containing serum on the apoptosis of chondrocytes induced by mechanics stimulus. Methods The BZD containing serum was extracted. The chondrocyte nutritive media was divided into 3 groups, i.e., the common nutritive medium group, the blank rabbit serum medium group, and the BZD nutritive medium group. The apoptosis of chondrocytes was in- duced by continuing mechanics stimulus in 24 h. Then the chondrocytes were collected. The apoptosis rate of chondrocytes was determined by flow cytometry. The contents of interleukin1β （IL-1β） and nitric oxide （NO） in the corresponding media were determined. Results The apoptosis of chondrocytes in the BZD nutritive medium group （19. 55 ±7.98）% was lower than that of the common nutritive medium group （39.32 ±13. 45）% and the blank rabbit serum medium group （37. 87 ±9.67）%, showing statistical difference （P〈0. 05）. The contents of IL-1β and NO were also lower in the BZD nutritive medium group with statistical difference when compared with those of the other two groups （ P 〈0.05）. Conclusion BZD containing serum could protect mechanics stimulus induced apoptosis of chondrocytes.