高容量血液滤过治疗对脓毒症伴急性肾衰竭犬血清及肝肺肾组织HMGB1、TNF-α、IL-6的影响

Effects of high volume hemofiltration on HMGB1,TNF-α,IL-6 in serum and live,lung,kidney tissues of sepsis dog model with acute renal failure

目的探讨高容量血液滤过(HVHF)治疗对脓毒症伴急性肾衰竭犬模型血清及肝、肺、肾组织炎症因子高迁移率族蛋白1(HMGB1)、TNF-α、IL-6的影响。方法 12只雄性Beagle犬双侧输尿管结扎后静脉注射内毒素(LPS)1.0mg/kg,制作脓毒症伴急性肾衰竭犬模型。随机均分为模型组和HVHF组(注射LPS后立即行HVHF治疗24h)。注射LPS前及注射后2、4、8、16、24h取静脉血检测血常规、肝肾功能、电解质,注射LPS前及注射后24h行动脉血气分析,ELISA法检测各时间点血清及注射后24h肝、肺、肾组织上清液中HMGB1、TNF-α、IL-6的浓度。结果与模型组相比,HVHF组血清HMGB1、TNF-α、IL-6的峰值及维持值降低,HMGB1达峰时间延迟,肝、肺、肾组织上清液中HMGB1、TNF-α、IL-6浓度降低,肝、肺、肾功能改善(P<0.05)。结论 HVHF可以降低脓毒症伴急性肾衰竭犬血清及肝、肺、肾组织炎症因子浓度,保护肝、肺、肾功能。

Objective To investigate the effects of high volume hemofiltration（HVHF） on high mobility group box chromosomal protein 1 （HMGB1）, TNF-a, IL-6 in serum and live, lung, kidney tissues of sepsis dog model with acute kidney failure. Methods The bilateral ureters of twelve male Beagle dogs were ligated and lipopolysaccharide（LPS） 1.0 mg/kg was injected to establish the sepstic dog models with acute kidney failure. The dogs were equally randomized to model group and HVHF group（treated with HVHF for 24 h after injection of LPS immediately）. Venous blood was collected tocheck blood routine, liver and kidney functions and electrolytes before and at 2,4, 8, 16,24 h after injection of LPS. Arterial blood gas analysis was performed before and at 24 h after injection of LPS. EILSA was used to determine the concentrations of HMGB1, TNF-a, IL-6 in serum at different time points above and in supernatant of liver, lung and kidney tissues at 24 h after injection of LPS. Results Compared with model group, the levels of HMGB1, TNF-a, I[-6 in serum and liver, lung, kidney tissues were decreased, peak time of HMGB1 level in serum was delayed, liver, lung and kidney functions were improved in HVHF group（P〈0. 05）. Conclusion HVHF therapy can decrease the concentrations of HMGB1,TNF-a and IL6 in serum and liver,lung and kidney tissues and protect the liver,lung and kidney functions of septic dog with acute kidney failure.