α-SMA和PCNA在不同缝合方法修复胆管中的表达

Expressions of α-SMA and PNCA in bile duct sutured by different techniques

目的探讨α-平滑肌动蛋白(α-SMA)和增殖细胞核抗原(PCNA)在手术修复后损伤胆管的表达。方法将15只兔随机均分为3组:空白对照组兔胆管正常;连续缝合组兔胆管切开后行连续缝合;间断缝合组兔胆管切开后行间断缝合。术后4周取胆管损伤处组织标本,用免疫组化超敏SP染色法检测α-SMA和PCNA的表达。结果术后4周时,两实验组较对照组α-SMA、PNCA的表达强度明显增高,连续缝合组又较间断缝合组α-SMA、PCNA的表达强度明显增高。(P<0.05)。结论相对于间断缝合,连续缝合的胆管瘢痕中肌成纤维细胞数量及细胞增殖程度持续处于更高水平,日后胆管发生瘢痕狭窄的可能性也更大。

ObjeeUve To investigate the expressions of a-smooth muscle actin（a-SMA） and proliferating cell nuclear antigen（PNCA） in the injured bile duct sutured by different techniques. Methods Fifteen rabbits were equally randomized into three groups. The injured bile duct in group A was closed with continuous suture,which in group B with interrupted suture. The rabbits in group C were taken as the blank controls. The rabbits were sacrificed on the 4th week after operation and the injured bile duct samples were examined for the expressions of a-SMA and PNCA tested by IHC SP staining. Results Compared to group C, the expressions of a-SMA and PNCA were higher in groups of A and B,which were higher in group A than those in group B（P^0. 05）. Conclusion The number and proliferation of myofibroblast cells in the injured bile duct are higher with continuous suture than interrupted suture,which results in a higher possibility of bile duct stenosis after operatiorl.