太极拳运动对中老年女性Th1/Th2平衡影响的机制研究

The Effects of Tai Ji Quan Exercise on the Th1/Th2 Balance in Middle-and Older-Aged Women

目的:从细胞因子角度在基因表达水平上探讨太极拳运动前后调节性T细胞(regulatoryT cells,Treg)对辅助性T细胞(helper T cell,Th)亚群Th1/Th2平衡的影响。方法:将自愿参加本实验的34名中老年女性随机分为太极拳组和对照组。太极拳组参加为期32周、每周3次、每次1小时的24式和42式太极拳锻炼;对照组不锻炼,保持原有生活状态。实验前后采集受试者外周血,使用流式细胞术检测Treg细胞数量;使用Real-time PCR定量分析Th1型淋巴细胞相关细胞因子干扰素-γ(Interferon-gamma,IFN-γ)和Th2型淋巴细胞相关细胞因子白介素-4(Interleukin-4,IL-4)、Treg相关细胞因子白介素-10(Interleukin-10,IL-10)和转化生长因子-β(transforming growth factor,TGF-β)以及细胞毒T淋巴细胞相关抗原-4(cytotoxic T lymphocyte-associated antigen-4,CTLA-4)mRNA表达。结果:实验后,太极拳组IFN-γ、TGF-β、CTLA-4和IL-10 mRNA表达量较实验前显著上升(P<0.01),IFN-γ与IL-4 mRNA表达量比值显著上升(P<0.05),Treg细胞占CD4+T细胞比例显著上升(P<0.01)。实验后,对照组除TGF-βmRNA表达下降(P<0.05)外,其他指标变化均无统计学意义。结论:太极拳运动影响外周血Treg细胞数量及相关细胞因子mRNA表达,提高中老年女性Th1/Th2型细胞因子mRNA表达量比值,在基因表达水平上改善老年女性Th1/Th2失衡。

Objective To investigate the effect of Tai Ji Quan（TJQ） exercise on the disequilibrium of Thl/Th2 in senior individuals. Methods Thirty four middle- and older-aged women were assigned to TJQ group （practicing TJQ for 34 weeks） and control group. Before and after the experiment,the mRNA expression of transforming growth factor-β （TGF-β）,interleukin-10 （IL-10）,cytotoxic T lymphocyte antigen 4 （ CTLA-4 ）, interleukin-4 （ IL-4 ）, interferon-γ （ IFN -γ）, and the number of regulatory T cells （Treg） in peripheral blood were tested using real-time polymerase chain reaction and flow cytometry, respectively. Results Before TJQ exercise,there were no significant differences in mRNA expression of TGF-β,CTLA-4,IL-10,IL-4 and IFN-γ,or the percentage of Treg cells in CD4＋ T cells between TJQ group and control group. After TJQ exercise,the mRNA expression of IFN-γ,TGF-β, CTLA-4 and IL- 10 were significantly increased in TJQ group（P 〈 0.01 ）. The IFN-γ/IL-4 ratio raised in TJQ group（P 〈 0.05）. And the percentage of Treg cells in CD4＋ T ceils in TJQ group was higher than control group（P 〈0.01 ）. Conclusions Long-term TJQ exercise enhances the number of Treg cells and the mRNA expression of cytokine and co-stimulatory molecule of Treg cells in middle- and older- aged women.