食管鳞癌组织FGF3和Podoplanin表达及与预后相关性分析

Expression of FGF3 and Podoplanin in esophageal squamous cell carcinoma and its relationship to prognosis

目的:探讨食管鳞癌组织中成纤维细胞生长因子3(FGF3)和Podoplanin表达及对预后的影响。方法:免疫组织化学法检测FGF3和Podoplanin在60例食管鳞癌组织中的表达,Cox回归模型和Kaplan-Meier生存曲线分析其表达与患者预后的关系。结果:在60例鳞癌组织中,FGF3的阳性率为92%(55/60),20例正常黏膜组织中的表达率为50%(10/20),差异有统计学意义,P<0.05。在食管鳞癌组织中,不同临床分期、分化程度、浸润深度、有或无淋巴结转移组之间FGF3蛋白的阳性率差异有统计学意义,P<0.05;不同临床分期、浸润深度、有或无淋巴结转移组和不同肿瘤大小组的淋巴管密度(LVD)的差异都有统计学意义,P<0.05。5年总生存率为35%,LVD计数>25/HP组5年生存率显著高于≤25/HP组,P<0.05。Cox模型多因素分析结果表明,肿瘤浸润深度、TNM分期和淋巴管密度均是影响生存期的独立因素,P<0.05。结论:FGF3是与食管鳞癌浸润和转移关系密切的指标。淋巴管密度,肿瘤浸润深度和TNM分期都是影响食管鳞癌患者预后的独立的危险因素。

OBJECTIVE： To investigate the expression of FGF3 and Podoplanin in esophageal squamous cell carcinoma（ESCC） and its effect on prognosis of patients. METHODS： Immunohistochemical method was employed to detect the expressions of FGF3 and Podoplanin in 60 cases of ESCC. The relationships between the expressions and prognosis of patients were evaluated by Cox regression model and Kaplan-Meier survival curve. RESULTS： The positive expression rate of FGF3 was 92% （55/60） in 60 cases of ESCC and 50% （10/20） in 20 cases of normal esophageal tissues. The differences had statistical significance（P〈0. 05）. Lymphatic vessel density（LVD） in ESCC was higher than that in normal esophageal tissues（P〈0. 05）. The differences of positive expression rate of FGF3 protein in different clinical stages, different degree of differentiation, different infiltration degree, and with or without lymph node metastasis in ESCC tissues had statistically significant（P〈0.05）. The differences of LVD in different clinical stages,different infiltration degree,with or without lymph node metastasis and different size of tumors in ESCC had statistical significance（P〈0. 05）. The 5-year overall survival rate of the patients in these cases was 35%. Univariate survival analysis indicated that the 5-year survival rate of patients with LVD count 〉25/HP was significantly higher than those with LVD count≤25/HP（P〈0. 05）. Cox multivariate analysis indicated that infiltration degree of ESCC,TNM staging,and LVD were all the independent prognostic factors af- fecting survival rate of patients（P〈0. 05）. CONCLUSIONS： FGF3 plays an important role in infiltration and metastasis of ESCC. LVD,TNM staging and infiltration degree were the independent prognostic factors affecting survival rate of patients with ESCC.