摘要
目的观察生长分化因子-5(GDF-5)对内侧副韧带(MCL)成纤维细胞增殖能力及胶原合成的影响。方法体外培养10周龄SD大鼠MCL成纤维细胞,分别加入不同浓度GDF-5,分为4组:0ng/mL组、10ng/mL组、50ng/mL组、100ng/mL组,用CCK-8法测定细胞增殖能力,羟脯氨酸测试盒测定细胞总胶原含量,逆转录-聚合酶链反应(RT—PCR)检测I与Ⅲ型胶原mRNA的表达。结果浓度为10、50、100ng/mL的GDF-5均能增强MCL成纤维细胞的增殖能力,以100ng/mL的GDF-5作用最显著,组间比较差异均有统计学意义(P〈0.05)。MCL成纤维细胞中加入GDF-5后,羟脯氨酸量增加,I型胶原mRNA表达增加,组间比较差异均有统计学意义(P〈0.05),但Ⅲ型胶原mRNA表达组间比较差异无统计学意义(P〉0.05)。结论GDF-5可以促进MCL成纤维细胞增殖及胶原合成进而促进韧带损伤愈合。
Objective To observe the effects of growth differentiation factor-5 (GDF-5) at different concentrations on the proliferation and synthesis of collagens of the fibroblasts from the medial collateral ligament (MCL) . Methods The MCL fibroblasts from ten-week-old SD rats were cultured in vitro with GDF-5 of different concentrations (0 ng/mL, 10 ng/mL, 50 ng/mL and 100 ng/mL, respectively) . The proliferation of fibroblasts was evaluated using the CCK-8 kit. The synthesis of total collagens was examined using the hydroxyproline kit. mRNA Expressions of collagen I and collagen III were assessed using RT-PCR. Results The addition of GDF-5 (10 ng/mL, 50 ng/mL and 100 ng/mL) remarkably enhanced the proliferation of MCL fibroblasts, compared with the null addition. The differences between the GDF-5 groups were significant, with the maximal proliferation in the 100 ng/mL group ( P 〈 0. 05). The addition of GDF-5 also enhanced the hydroxyproline (HYP) content and the expression of collagen I, with significant between-group differences ( P 〈 0. 05), but did not increase the expression of collagen III ( P 〉 0. 05) . Conclusion Since GDF-5 can enhance the proliferation and collagen synthesis of the MCL fibroblasts, it can enhance MCL healing.
出处
《中华创伤骨科杂志》
CAS
CSCD
北大核心
2012年第6期523-526,共4页
Chinese Journal of Orthopaedic Trauma
基金
山东省科技攻关计划项目(2008GGl0002001)
关键词
成纤维细胞
细胞增殖
内侧副韧带
生长分化因子-5
Fibroblasts
Cell proliferation
Medial collateral ligament
Growth differentiation factor-5