医用几丁糖对增生性玻璃体视网膜病变抑制作用的研究

Study of the preventing effect of Chitosan on proliferative vitreoretionopathy

目的探讨医用几丁糖对增生性玻璃体视网膜病变的抑制作用。方法40只健康新西兰大白兔，随机分5组：正常对照组；模型组（玻璃体腔内注射0．1ml生理盐水和0．1ml自血）；实验1、2、3组（玻璃体腔内分别注射5mg／ml、10mg／ml、20mg／ml的医用几丁糖0．1ml和0．1ml自血）。用裂隙灯显微镜、眼底镜、B超观察记录玻璃体视网膜的情况。于术后第28天定量检测玻璃体腔内bFGF和IL-6的含量并用HE染色法，光镜下观察视网膜组织结构。结果观察玻璃体视网膜增生级别，第14、21、28天实验3组均显著低于模型组及另2个治疗组（P〈0．05）。实验1、2、3组中bFGF和IL-6的含量均显著低于模型组（P〈0．05）；实验2、3组bFGF和IL-6的含量与实验1组比较均有差异（P〈0．05）；结论医用几丁糖能够有效的抑制增生性玻璃体视网膜病变中bFGF和IL-6表达，能够有效的防治增生胜玻璃体视网膜病变。

Objective To provide experimental reason to prevent proliferative vitreoretinopathy （PVR） with chitosan. Methods Forty New Zealand white rabbit were selected and were divided into five groups in random. Normal group was not injected any medicine in vitreous body, model group was injected saline 0.1ml and 0.1ml blood, experiment group 1, 2, and 3 was injected different con- centration chitosan （Smg/ml, 10mg/ml, 20mg/ml） with 0.1ml and 0.1ml blood. Changes in vitreous body and retina were observed with silt lamp micoroscope, funduscope and B ultrasound. The eyes were enucleation to make samples for optic microscope for observing pathological changes of the ret- ina on 28 days after injection of drug. The content of bFGF and IL-6 was measured by ELISA and RIA. Results Hyperplasia level of vitreous body and retina in experiment all 3 groups was lowest than model group and other two groups on the day 14, 21 and 28 （P〈0.05）. The content of bFGF and IL-6 in the experiment groups was decreased significantly compared with control group statistical- ly （P〈0.05）. The content of bFGF and IL-6 in the experiment group 2 and 3 was different com- pared with experiment group 1 statistically （P〈0.05）. Conclusions Chitosan can decrease the content of bFGF and IL-6 in the vitreous body, and then prevent the proliferative vitreoretinopathy.