淋巴管形成及组织蛋白酶-D的表达与喉癌转移的关系

The function of lymphangiogenesis and the expression of Cathepsin D in laryngeal carcinoma metabasis

目的:研究淋巴管形成及组织蛋白酶-D(Cath-D)的表达与喉癌颈淋巴结转移及临床病理因素的关系。方法:应用免疫组织化学SP法检测Cath-D在喉癌组织中的表达情况;使用肾小球足突细胞膜蛋白作为淋巴管内皮标记物,标记喉癌组织中淋巴管并测量淋巴管密度,以其癌旁正常组织作为对照。结果:①Cath-D的阳性表达率在正常黏膜、喉癌组织以及在不同病理分级、临床分期、有无颈淋巴结转移中有显著性差异;而在年龄及临床分型分组中无明显差异。②毛细淋巴管密度在癌周正常组织与癌旁以及在临床分期、临床分型、有无颈淋巴结转移中有显著性差异;而在年龄及病理分级分组中无明显差异。结论:①微淋巴管密度增高及Cath-D表达增高能促进喉癌的颈淋巴结转移;②微淋巴管密度增高和Cath-D表达增高在喉癌组织中有一定相关性,在喉癌淋巴结转移中具有协同作用。

Objective：To study the function of lymphangiogenesis and the＇expression of Cathepsin D（Cath-D） in laryngeal carcinoma metabasis and clinical pathology character. Method：The expression of Cath-D were detected in 76 laryngeal carcinoma with immunohistochemistry（SP method）. Podoplanin was used as the marker of lympgatic vessel endotheliocytes to label lympgatic vessel in 76 laryngeal carcinoma,lymphatic microvessel density were measured,and the paraneoplastic tissues was used as control group. Result：（1）The positive rate of Cath-D in paraneoplastic tissue,laryngeal carcinoma and in pathology classification,in clinical stage, in cervicale lymphonode metastasis negative and positive group were significantly different. However, there had no difference between the positive rate of Cath-D in the agespecific and clinical classification. （2）The lymphatic microvessel density in paraneoplastic tissue,laryngeal carcinoma and clinical stage, in glottic carcinoma and supraglottic carcinoma, in cervical lymphonode metastasis negative and positive group were significantly different;but there had no difference in agespecific and pathology classification. Conclusion： （1）The high expression Of lymphatic microvessel density and the increasing expression of Cath-D could promote cervical lymphonode metastasis in aryngeal carcinoma. （2）There had a correlation between the high expression of lymphangiogenesis and Cath-D in laryngeal carcinoma, and had cooperation in a ryngeal carcinoma lymphonode metastasis.