摘要
目的 :探讨大鼠和猴iv、im重组人碱性成纤维细胞生长因子 (rh -bFGF )的药动学特征。方法 :大鼠iv125I-rh -bFGF1600ng/kg,猴分别iv和im125I-rh-bFGF750ng/kg 后 ,用SDS -PAGE结合放射性计数测定生物样品中rh-bFGF含量。结果 :rh -bFGF在大鼠和猴血浆中的T1/2 较短 ,在大鼠肺和坐骨神经的放射性计数最高 ,48h尿累积排出降解物为给药量的92 52 %。结论 :大鼠和猴iv125I-rh-bFGF后 ,血中消除快 ,降解产物主要由肾脏排出 ,主要分布于大鼠在肺和坐骨神经。猴im给药的绝对生物利用度为90 %。
AIM:To study pharmacokinetic characteristics after iv and im injection of recombinant human basic fibroblast growth factor(rh-bFGF)to rats and monkeys.METHODS:125I-rh-bFGF 1 600ng/kg was intravenously administered to 10 rat_s.125I-rh-bFGF 750ng/kg were intravenously and intramuscularly given to 3 monkeys separately.SDS-PAGE and radioactive_count were used to determine the rh-bFGF concentrations in biological samples.RESULTS:T1/2α of rh-bFGF in rats and mon_keys were rather short.Radioactive counts of the lungs and sciatic nerves in rats were the highest.A ccumulated degradation products in 48h urine were 92 52% of dose given in rats.CONCLUSIONS:Prototype in blood eliminated rapidly and a majority of degradation products excreted via kidneys after iv injection of 125I-rh-bFGF to rats and monkeys.rh-bFGF mainly distributed in sciatic nerves after iv administration to rats.Absolute bioavailability of im administration to monkeys was 90±5%.
出处
《中国药房》
CAS
CSCD
2000年第2期62-64,共3页
China Pharmacy
