摘要
研究溶剂汽油对大鼠皮肤皮脂成分的影响。 [方法 ]45只 Wistar雄性大鼠分为对照组、实验组和干预组 ,染毒方式为经皮染毒。实验组染毒剂量分别为 :2 5 0 m g/ cm2 × 1 d,2 5 0 mg/ cm2 × 4d,2 5 0 m g/ cm2 × 8d,染毒面积为 2× 3cm2 。干预组则是涂抹防护剂后 ,再进行染毒 ,染毒剂量 2 5 0 mg/ cm2 × 8d。染毒结束后 ,分离染毒部位皮肤 ,采用高效薄层层析法 (HPTL C)测定皮脂。[结果 ]各实验组大鼠皮肤的表皮皮脂总量明显低于对照组 ,干预组与对照组相比无统计学差异 ;实验组大鼠神经酰胺、游离脂肪酸和胆固醇的含量也有不同程度的降低。 [结论 ]溶剂汽油可降低皮肤皮脂含量 ,并破坏皮脂的正常构成。
Objective] To investigate lipid metabolism disturbance caused by gasoline on skin from rat and the mechanism.[Methods] 45 male Wistar rats were divided into control group,3 different exposure level groups,which exposed to gasoline on 2cm×3cm skin area with the dose of 250mg/cm 2 for 1 (group A),4 (group B) and 8 days (group C),respectively,and an intervention group exposed to gasoline of 250mg/cm 2 for 8 days after the application of protective agent on skin.After the treatment,departed skin from intoxicated area.Epidermis was separated from rat skin,and lipids were extracted from desiccated epidermis,which was weighted.Contents of cholesterol,fatty acid and ceramide were measured with HPTLC.[Results] Lipids of epidermis in all exposure groups were significantly decreased compared with control group ( P <0.05 or P <0.01);there were no obvious changes of lipid content in the intervention group.[Conclusion] The lipids of skin,including ceramide,fatty acid and cholesterol were decreased in rat derma exposed to gasoline.
关键词
溶剂汽油
皮脂分析
皮肤毒理
gasoline
corneum lipids
dermatotoxicology
