催产素受体在雌性山羊腹腔肠系膜前神经节的分布

Immunohistochemical Localization of OTR on Celiac Superior Mesenteric Ganglion in Female Goats

本研究旨在探讨催产素(OT)是否可以影响雌性山羊腹腔肠系膜前神经节的活动。采用免疫组化SP法观察催产素受体(OTR)在雌性山羊腹腔肠系膜前神经节的分布特点。结果显示,催产素受体在腹腔肠系膜前神经节分布广泛,节内的神经细胞、支持细胞和过路纤维均有OTR免疫阳性产物分布;OTR主要在神经细胞中表达,相对表达量与其他非神经结构相比差异性极显著(P<0.01);在神经细胞中,OTR免疫阳性产物主要存在于胞膜和胞质,核不着色。雌性山羊腹腔肠系膜前神经节神经元对OT具有反应性,提示OT可能通过影响腹腔肠系膜前神经节神经元的活动,从而经其发出的交感节后神经这一途径调节胃肠的生理活动。

In order to determine the distribution of the oxytocin receptor （OTR） on celiac superior mesenteric ganglion in female goats, we established an immunohistochemical assay in female goats using SP method as the detecting measure of OTR localization in celiac superior mesenteric ganglion in female goats. Our results indicated that OTR immunoreactive substances were dis- tributed in nerve cells, supporting cells and crossing fiber. OTR mainly expressed in the nerve cells, the relative expression of immunoreactive product OTR was extremely significantly com- pared with other non-nerve cells （P〈0. 01）. In nerve cells, OTR immunoreactive substances were distributed mainly in the membrane and cytoplasm, but not in the nuclear. The results sug gested that neurons in superior mesenteric ganglion had reaction with OT in female goats and OT might regulate the functional activities of the gastrointestinal system by sympathetic nerve from celiac superior mesenteric ganglion.