颈上交感神经节脑内移植治疗帕金森病的实验研究

Transplantation of adult allogeneic superior cervical ganglia into the brain of rat model of Parkinson’s disease

目的 :研究治疗帕金森病的理想脑内移植物。方法 :建立 SD大鼠帕金森病动物模型 ,选取 SD模型大鼠30只分成 3组 (移植组 12只 ,对照组 12只 ,空白组 6只 )用同种成年大鼠颈上交感神经节 (SCG)制成细胞悬液 ,用微量注射器、立体定向仪将细胞悬液植入移植组模型鼠毁损侧尾核头部。每 2周观察动物旋转行为变化。 6个月后处死动物 ,制成脑切片 ,行 TH免疫组化及 HE染色 ,切片光镜观察。结果 :SCG移植后 6个月 ,移植组大鼠旋转行为明显减少。组织学观察发现移植区有 TH样阳性细胞存活 ,且存活数与治疗效果成正比。结论 :同种异体成年大鼠的 SCG脑内移植 ,移植物能存活并对帕金森病模型鼠有短期治疗作用。

Objective: In order to find out evidence in explicating whether the transplanted allogeneic adult superior cervical ganglion (SCG) cells can surviving in the brain and to find out an ideal graft for the surgical treatment of Parkinson's disease. Method:To produce Parkinson's disease rat model, 30 models were selected and divided into three groups, a SCG transplant group (n=12); a control transplant group (n=12) and a lesion only group (n=6). Allogeneic SCG cell suspension were stereotaxically transplanted into the head of the caudate-putamen of the rat of ST group. The supernatant of SCG cell suspensions were transplanted into the head of the caudate-putamen of the rat of ET group. Non-grafted lesioned rats served as control group. 24 weeks after grafting, all of the rat were sacrificed for HE and Tyrosine hydroxylase immunohistochemistry staining. Results: By 2 weeks post grafted, ST and ET group rat models mean rotate frequency caused by apomorphine was get down.By 24 weeks post grafted, the mean rotating frequency of three groups was list as: ST group 3.18±2 75、ET group 16.36±2 20、CT group 18.16±1.17. There has significant difference between ST and ET. TH-like immuno-reactive neurons were found in the transplanted areas, along with this, the graft-derived TH positive fiber were found around the grafted area. Conclusion: 1. The transplantation alone only have a short period therapeutical effect on Parkinson's disease. 2. Allogeneic SCG cells transplantation can survive in host environment and ameliorate behavioral deficits of the host.