摘要
目的:采用高效液相色谱(HPLC)法测定裸花紫珠中毛蕊花糖苷的含量。方法:采用ZORBAX SB C18(4.6 mm×250 mm,5μm)色谱柱,流动相为0.1%磷酸溶液:乙腈(77∶23),流速1.0 mL.min-1,柱温30℃,检测波长为334 nm,进样量取10μL。理论板数为6 193,分离度为4.1。结果:毛蕊花糖苷浓度在10.18~402.39μg(r=0.999 9,n=7)范围内线性关系良好,平均回收率(n=6)为96.13%,RSD=1.38%。结论:本研究方法专属性强、重复性好、结果准确,为裸花紫珠中毛蕊花糖苷含量测定提供了科学、专属的方法。
OBJECTIVE: To determine the content of verbascoside in callicarpa nudifloraby HPLC.METHODS: The chromatographic column ZORBAX SB C18(4.6 mm×250 mm,5 μm) was used with column temperature maintained at 30 ℃.The mobile phase consisted of 0.1% phosphoric acid solution-acetonitrile(77∶23) with flow rate of 1.0 mL·min-1,detection wavelength of 334 nm and sample size of 10 μL.The number of the theoretical plates were 6193 and the resolving power was 4.1.RESULTS: The linear range of verbascoside was 10.18-402.39 μg(r=0.999 9,n=7) with an average recovery rate of 96.13%(n=6,RSD=1.38%).CONCLUSION: The method is specific,reproducible and accurate in result,and it is a scientific and specific method for determining verbascoside content in callicarpa nudiflora.
出处
《中国医院用药评价与分析》
2012年第5期438-439,共2页
Evaluation and Analysis of Drug-use in Hospitals of China