细胞凋亡和P53在宫颈癌及宫颈上皮内瘤变中的表达

Apoptosis and expression of p53 gene in cervical intraepithelial neoplasia and squamous cell carcinoma.

目的：探讨细胞凋亡和P53在宫颈癌及宫颈上皮内瘤变中的作用。方法：用DNA缺口末端标记技术(TUNEL)和免疫组化技术原位观察15例正常宫颈、22例CIN*"I/Ⅱ、16例CINⅢ和52例浸润性宫颈鳞癌(ISCC)组织中细胞凋亡标记指数(A-LI)、PCNA(增殖细胞核抗原)标记指数(PCNA-LI)和P53表达阳性率及P53标记指数(P53-LI)。结果：在正常宫颈上皮未检测到凋亡细胞，随着CIN病变的进展，A-LI增高(P＜005)，在ISCC，A-LI明显低于CIN(P＜001)；PCNA-LI随宫颈癌各级病变的进展逐渐增高(Ｐ＜005)；在正常宫颈和CINI/Ⅱ均未检测到P53表达，在CINⅢ，P53-LI明显低于ISCC(P＜001)。结论：细胞凋亡在宫颈癌发生发展过程中发挥重要作用，P53蛋白的积聚可能对细胞凋亡起抑制作用。

objective：To explore the effects of apoptosis and p53 gene i n cervical intraepithelial neoplasia and squamous cell carcinoma.Methods : The apoptotic labeling indices(A-LI),PCNA(proliferating cell nuclear ant igen)-LI,P53-LI and the positive expression of P53 protein were observed in 15 c ases of normal cervical epithelium,22 cases of CIN I/Ⅱ，16 cases of CINⅢ and 5 2 cases of ISCC(invasive squamous cervical carcinoma)tissues,using DNA nick end l abeling technique and immunohistochemical staining for PCNA and P53 proteins.Results:No apoptotic cell was observed in normal cervical epitheliu m.A-LI increased with the development of CIN(P＜005).A-LI in ISCC were significantly lower than those in CIN(P＜001).PCNA-LI gradual lincreased with the development of every lesion grade of cervical carcinomas(P＜005).P53 protein was not detected in normal cervical epithelium an d CIN I/Ⅱand P53-LI in CINⅢ were significantly lower than those in ISCC(P ＜001).Conclusions:Apoptosis play an important role in t he development of cervical carcinoma and P53 protein may block apoptosis.