宫颈癌患者血清TNF-α、STNFR水平及其与临床关系的研究

Study of the correlation between clinical feature and serum TNF-α,STNFR level of cervical carcinoma patients

目的：探讨肿瘤坏死因子-α(TNF-α)、可溶性肿瘤坏死因子受体(STNFR)与宫颈癌发生、发展及淋巴转移的关系。方法：用双抗体夹心ELISA法检测宫颈癌42例和宫颈上皮内瘤变(CIN)14例血清TNF-α和STNFRⅠ、STNFRⅡ，并与正常34例对照。结果：宫颈癌患者血清TNF-α、STNFRⅠ、STNFRⅡ以及CIN患者STNFRⅠ、STNFRⅡ水平较正常对照组明显增高(P＜001)；Ⅱ期宫颈癌患者血清STNFRⅠ、STNFRⅡ水平与Ⅰ期患者相比，差异无显著性；宫颈癌有淋巴转移组患者血清STNFRⅠ、STNFRⅡ水平明显高于无淋巴转移组(P＜00001)；经相关分析STNFRⅠ、STNFRⅡ在宫颈癌、宫颈癌淋巴转移组、宫颈癌无淋巴转移组及正常对照组均呈正相关关系，仅CIN组无明显正相关。结论：TNF-α、STNFR与宫颈癌的发生发展有关，STNFR可能是判断宫颈癌是否发生淋巴转移较好的监测指标，对判断预后有重要价值。

Objective:To explore the correlation among tumor necrosis f actor-α(TNF-α),soluble tumor necrosis factor receptor (STNFR) and the occurren ce, progress,lymphatic metastasis of cervical carcinoma.Methods:TNF- α,STNFRⅠ,STNFRⅡ were measured by sandwich ELISA in 42 cases of patients with cervical carcinoma,14 cases with cervical intraepithelial neoplasm(CIN) and 34 c ases of normal women as the control.Results:The serum TNF-α,STN FRⅠ,STNFRⅡ of cervical carcinoma patients and STNFRⅠ,STNFRⅡ of CIN patients were significantly higher than those in the control group(P＜001),STNFRⅠ and STNFR Ⅱ in cervical carcinoma patients with lymphatic metastasis were significantly h igher th an those without lymphatic metastasis(P＜001).There was a signifi cant positive correlation between STNFRⅠand STNFRⅡin cervical carcinoma group and the control group.Conclusions:TNF-α,STNFR are associated with the o ncogenesis and development of cervical carcinoma.There are important practical v alues to use STNFR as an index of lymphatic metastasis in judging prognosis.