白血病端粒酶检测与临床观察

Test of Telomerase Activity of Acute Leukemia and Clinical Observation

目的 :检测急性白血病端粒酶与临床的关系。方法 :端粒重复扩增法结合吸光度测定及临床病例分析。结果 :各种类型急性白血病均表现高的端粒酶活性 ,伴有异常染色体核型或高细胞白血病病例具有明显高的活性。结论 :急性白血病端粒酶活性与异常染色体核型和病情相关 ,检测白血病细胞端粒酶 。

Objective:Test of telomerase activity of acute leukemia and exploringits clinical significance.Methods:The telomeric repeat amplification protocol(TRAP)combined with densitometry and clinical examination were performed in leukemia patients.Results:The strong telomerase activity was observed in presence of acute lymphoblastic leukemia and myeloid leukemia. The telomerase activity of the patients with abnormal chromosomes was higher than that of the cases with normal karyotype.The patients with high leucocyte count showed high telomerase activity.Conclusion:The acute leukemia has high telomerase activity,which is related to abnormal karyotyp and clinical findings.The analysis of telomerase acticity might be significant in diagnosis and treatment of leukemia.