高效液相色谱法测定甲硝唑的唾液浓度

Determination of metronidazol in saliva by HPLC

本文报告用反相高效液相色谱法测定人体唾液中甲硝唑含量的方法。服药后的唾液用高氯酸去蛋白后直接进弹测定,紫外检测波长为320nm,采用外标法定量。本法的灵敏度为2ng/ml,标准曲线的线性迭40μg/ml。本法的回收率为为95.23～101.5%,平均97.69%,批内CV%=1.32,批间CV%=3.25。本法可有效地应用于甲硝唑的临床药理学研究。

Metronidazol in saliva was determined by HPLC,with detection by ultravi-olet absorbance at 320 nm.External standard method is used for determinationThe sensitivity is 2ng/ml.The standard curve is linear up to 40ug/ml.The aver-age recovery were 97.69%(95.23～101.50%).The intrabatch and inter-batch coeffici-ents of variation were 1.32% and 3.25% respectively.The method was rapid,simple,accurate,less interfered and has been used to determine metronidazol insaliva for pharmacokinetic and therapeutic concentrations studies