胃液与胃粘膜中庆大霉素的测定及临床意义

Monitoring on the concentration of gentamicin in gastric fluid and gastric antrum mucosa with fluorescence polarization immunoassay

本文采用荧光偏振免疫分析法(简称FPIA)测定55例胃疾病患者的胃液、血清、胃窦粘膜中庆大霉素的浓度,结果表明,口服庆大霉素后经较长时间在胃液和胃窦粘膜组织中,可保持较高的有效浓度,其中18例口服庆大霉素4万U 后12h 胃液浓度为50.33±12.64μg/ml;4例24h 胃液庆大霉素浓度为46±6.6μg/ml;25例患者口服庆大霉素4万U 后12h 胃窦粘膜浓度为3.17±1.24ug/g,而血清中庆大霉素未检出。这项工作为临床合理使用庆大霉素提供了科学依据。

We monitored the concentration of gentamicin in gastric fluid,serum and gastricantrum mucosa in 55 patients with stomach diseases using Fluorescence Polariza-tion Immunoassay(FPIA).Our results showed that there is a higher effectiveconcentration of gentamicin in gastric fluid and gastrio antrum mucosa after along time of oral administration.The average gastric fluid concentration in 18patients after 12 hours of gentamicin is 50.33ug/ml(SD 12.64);in 4 patients after24 hours is 46μg/ml(SD6.6);the average concentration in gastric antrum mucosa in25 patients after 12 hours 3.17μtg/g(SD1.24).We couldn't any gentamicin in serum.