肌肽对高糖环境下心肌成纤维细胞胶原表达的影响

The Effect of Carnosine on Collagen Content in Rat Cardiac Fibroblast Under High Glucose Condition

目的:探讨肌肽对高糖环境下心肌成纤维细胞中胶原生成的影响及作用机制。方法:原代培养心肌成纤维细胞,将细胞分为正常糖组(NG,5.5mmol/L glucose)、高糖组(HG,25mmol/L glucose)、高糖+10mmol/L肌肽组、高糖+20mmol/L肌肽组、高糖+40mmol/L肌肽组、高糖+SB组(HG+10μmol/L SB203580)、高糖+PD组(HG+10μmol/L PD98059)。ELISA检测胶原Ⅰ、Ⅲ的含量,Western blot检测TGF-β1、p-p38 MAPK和p-ERK(1/2)等蛋白的表达。结果:与正常糖组相比,高糖组中胶原Ⅰ和胶原Ⅲ含量增加(P<0.01);TGF-β1、p-p38和p-ERK等表达增加(P<0.01);与高糖组相比,高糖+肌肽组中胶原Ⅰ、Ⅲ、TGF-β1、p-p38、和p-ERK等均降低(P<0.05);高糖+SB组和高糖+PD组中胶原Ⅰ、Ⅲ表达减少(P<0.05)。结论:肌肽对心肌成纤维细胞中胶原生成具有抑制作用,且通过MAPK通路实现。

Objective： To investigate the effect and mechanism of carnosine on collagen content in rat cardiac fibroblasts under high glucose condition.Methods： The rat cardiac fibroblasts were divided into seven groups,NG group（5.5mmol/L glucose）,HG group（25mmol/L glucose）,HG＋10mmol/L carnosine group,HG＋20mmol/L carnosine group,HG＋40mmol/L carnosine group,HG＋SB group（HG＋10 μmol/LSB203580）,HG＋PD group（HG＋10 μmol/L PD98059）.The collagenⅠand Ⅲ contents were detected by ELISA,and Western blotting was used to detect the protein expression level of TGF-β1,p-p38 MAPK and p-ERK（1/2）.Results： By the end of the experiment,compared with NG group,the expression level of collagen Ⅰand collagen Ⅲ were significantly higher in the HG group（P〈0.01）;The protein expression level of TGF-β1,p-p38,p-ERK were also significantly higher in the HG group（P〈0.01）.However,after supplement the carnosine,the level of collagen Ⅰ,collagen Ⅲ,TGF-β1,p-p38 and p-ERK were significantly reduced in HG＋Car group than HG group（P〈0.05）;Compared with HG group,the collagen Ⅰand Ⅲ were also significantly decreased in HG＋SB group and HG＋PD group.Conclusion： Carnosine reduced collagen production under high glucose via MAPK signaling pathway.