肝细胞癌患者外周血HSF1基因表达的检测及应用价值

Development and Application of Real-time Quantitative Polymerase Chain Reaction for the Detection of Human Hepatocellular Carcinoma Related Novel Gene HSF1 Expression

目的:建立检测HSF1 mRNA的real-time PCR的方法,了解肝细胞癌患者外周血中HSF1的表达水平及其与各临床病理特征之间的关系。方法:利用real-time PCR的方法检测20例肝细胞癌患者及20例正常人群外周血中HSF1 mRNA的表达量。结果:肝细胞癌患者外周血中的HSF1 mRNA表达量显著高于正常人群(P<0.05);肝细胞癌患者外周血中HSF1 mRNA的表达水平在不同性别、肿瘤大小、门静脉侵犯情况、HbsAg水平及AFP水平的患者中的差异无统计学意义(P>0.05);在不同病理分化程度、TNM分期的患者中的差异有统计学意义(P<0.05)。结论:real-time PCR技术可以成功检测外周血中HSF1 mRNA的表达量,HSF1可能与肝细胞癌的发生发展密切相关。

Objective： To establish a quantitative real-time PCR method for detection of HSF1 gene expression level in human hepatocellular carcinoma and to investigate the relationship between HSF1 mRNA expression level and the clinicopathological characters.Methods： Real-time PCR was employed to determine the expression level of HSF1 mRNA in peripheral blood of 20 specimens of human hepatocellular carcinoma patients and normal human.Results： Comparing with normal human,HSF1 present higher expressions in human hepatocellular carcinoma patients（P〈0.05）.There is no statistical differences of HSF1 mRNA expression level between the group with different sexes,tumour diameters,portal involvement levels,HBsAg levels and AFP levels（P〉0.05）.There is statistical differences of HSF1 mRNA expression level between the group with different differentiations and TNM stages（P〉0.05）.Conclusion： The established quantitative real-time PCR method can successfully detect the expression level of HSF1 mRNA,which may be closely related to the occurrence and development of human hepatocellular carcinoma.