二乙基亚硝胺诱发C3H/HeN小鼠肝癌模型的研究

Establishment of DEN-induced Hepatocarcinoma Model in C3H/HeN Mice

[目的]建立C3H/HeN小鼠诱发性肝癌动物模型,为研究肝癌发病机理及开展肝癌药物治疗提供小鼠诱发性肝癌模型。[方法]取20只C3H/HeN小鼠作为模型组,每日喂予含30μg.mL-1二乙基亚硝胺(DEN)的饮水,连续23周后,观察其肝癌的发生情况。另取6只C3H/HeN小鼠作为正常组。[结果]造模23周后,模型组小鼠血清中ALP、ALT、AST、TBIL、肝脏指数、结节数和γ-GT活力显著增加(P<0.01),小鼠体重、血清中TP、ALB、BUN、CREA和肝脏中TP含量显著降低(P<0.01),存活15只小鼠中有13只肉眼能观测到肝癌发生,肝癌发生率为86.67%,小鼠肝脏表面较多结节,体积增大,表面粗糙、色彩暗淡,多个大小不一的灰白色圆形结节,组织学类型为肝细胞癌,其中以梁状型和腺样型居多。[结论]应用DEN诱发的小鼠肝癌,与人肝癌的发生过程相似,是一种较为理想的诱发性肝癌动物模型,但具体实验方法需进一步改良和研究。

[Objective] Establish DEN-induced hepatocarcinoma model in C3H/HeN mice, provide induced liver cancer model in mice for studying hepatocellular carcinoma pathogenesis and carry out the treatment of liver cancer drug. [Methods] Twenty C3H/HeN mice were induced by administering 30 μg/mL DEN water for 23 weeks; observe the occurrence of liver cancer. Take another six C3H/HEN mice as normal control. [Results] Induced after 23 weeks, the serum ALP, ALT, AST, TBIL level and fiver Inde, nodules, γ-GT increased significantly in mice（P〈0.01）. Weight, serum TP, ALB, BUN, CREA and liver total protein content decreased significantly in mice. Thirteen mice had fiver cancer by naked-eye observations of survival 15 mice, fiver cancer incidence rate of 86.67%. More nodules of the liver surface in mice, the volume increased, surface roughness, color dim gray rounded nodules of several different sizes, histological type of HCC, in which the majority was of the trabecular type and adenoid type. [Conclusion] Application of DEN-induced mouse liver cancer, for the occurrence of human hepatoma, is an ideal induced liver cancer animal models, but the specific experimental methods need further improvement and research.