超低氢氰酸高丹草新品系选育及ISSR分子标记验证

Breeding of New Strains of Sorghum-Sudangrass of Super-low Hydrocyanic Acid Content and ISSR Molecular Marker Verification

为培育氢氰酸含量极低、综合农艺性状优良的高丹草新品种,利用ISSR分子标记辅助选择技术对散穗高粱与黑壳苏丹草、白壳苏丹草、棕壳苏丹草、红壳苏丹草4个杂交组合后代进行选育研究。首次选育出综合农艺性状优异、鲜草氢氰酸含量极低(株高约100cm时,鲜草氢氰酸含量仅为2.58～4.77mg/kg)的散穗高粱—黑壳苏丹草、散穗高粱—白壳苏丹草、散穗高粱—棕壳苏丹草、散穗高粱—红壳苏丹草新品系。散穗高粱—黑壳苏丹草、散穗高粱—白壳苏丹草杂交组合的新品系分别含有与超低氢氰酸含量相关的ISSR特征片段H-1、H-2、H-3和B-1、B-2、B-3,利用分子标记技术所选择的目标性状准确、可靠、遗传稳定。

In order to breed new strain of Sorghum-Sudangrass of super-low hydrocyanic acid content and good agronomic traits,the ISSR molecular marker technology was used in study on breeding four hybrid offsprings via crossing between loose spike Sorghum and black hull Sudangrass,white hull Sudangrass,brown hull Sudangrass,red hull Sudangrass.Four new strains of Sorghum-Sudangrass with excellent agronomic traits and super-low hydrocyanic acid content in fresh grass（HCN content just was only 2.58~4.77mg/kg in fresh grass when the height was 100cm）,loose spike Sorghum-black hull Sudangrass,loose spike Sorghum-white hull Sudangrass,loose spike Sorghum-brown Sudangrass,loose spike Sorghum-red Sudangrass,were obtained firstly.The two new strains of loose spike Sorghum-black hull Sudangrass and loose spike Sorghum-white hull Sudangrass had some characteristic ISSR fragments associated with low HCN content,which were H-1,H-2,H-3 and B-1,B-2,B-3.The target traits associated with molecule markers was accurate,reliable,and genetically stable.