甘薯叶黄酮对Ⅱ型糖尿病模型小鼠的降糖及DNA损伤修复作用

Hypoglycemic and repaired DNA damage effects of flavonoids of Ipomoea batatasleaves on type Ⅱ diabetes mellitus mice model

【目的】探讨甘薯叶黄酮(FIBL)的降糖作用及其对糖尿病DNA损伤的修复作用。【方法】采用高糖高脂日粮和链脲佐菌素(STZ)建立Ⅱ型糖尿病(NIDDM)小鼠动物模型,然后将其分为模型对照组(DC)、FIBL低剂量组(FTL)、FIBL高剂量组(FTH)和罗格列酮组(RT组),另取正常小鼠作为正常对照组(NC)。其中,正常对照组、模型对照组小鼠每天灌胃0.2mL dH2O,FIBL低剂量和高剂量组小鼠每天分别灌胃75和150mg/kg的FIBL,RT组每天灌胃3mg/kg罗格列酮,连续灌胃28d。对小鼠一般情况进行观察,并于灌胃0(灌胃前),7,14,21,28d尾静脉采血检测血糖;29d时,断头采血检测血脂,并分离淋巴细胞,采用单细胞凝胶电泳技术(SCGE)检测DNA损伤情况。【结果】动物模型建模成功后,Ⅱ型糖尿病(NIDDM)小鼠存在多饮、多尿、多食,及一定程度的胰岛素抵抗等情况。FIBL治疗结果显示,FIBL能控制NIDDM小鼠体质量的增长;能有效降低NIDDM小鼠的血糖、血脂水平;对NID-DM小鼠的DNA损伤具有修复作用。【结论】甘薯叶黄酮能改善高血糖症状及脂质代谢紊乱状况;可以治疗和延缓NIDDM并发症的发生。

【Objective】 Hypoglycemic effect of flavonoids of Ipomoea batatas leaves（FIBL） and its effect on the repair of DNA damage of diabetes mellitus were studied.【Method】 The high sugar and high fat diet and streptozotocin（STZ）-induced type Ⅱ diabetes mellitus（non-insulin dependent diabetes mellitus,NIDDM） mice model were used,and then divided into model control group,FIBL low-dose group,FIBL high-dose group and rosiglitazone group.Some normal mice were taken as normal control group.Mice of model control group and normal control group were daily fed 0.2 mL dH2O,respectively;mice of FIBL low-dose group and high-dose group were daily fed 75 and 150 mg/kg FIBL respectively and mice of RT were daily fed 3 mg/kg rosiglitazone,all for 28 d continuously.Observation of animals in general was carried out,and blood of tail vein was collected to measure glucose after 0,7,14,21,28 d.On 29 d,blood of heads was collected to measure lipids and lymphocytes were separated,and then single cell gel electrophoresis（SCGE） was used to test DNA damage.【Result】 After the animal models had been successfully set up,NIDDM mice showed some symptoms,including polydipsia,polyuria,polyphagia and a certain degree of insulin resistance.Treatment results of FIBL showed that FIBL could control NIDDM mice from increasing body weight,decreasing blood glucose and blood lipid levels.FIBL could repair DNA damage of NIDDM mice.【Conclusion】 Flavonoids of Ipomoea batatas leaves can improve the symptoms of high blood sugar and lipid metabolism,can treat and delay the occurence of NIDDM complication.