甘蓝一代杂种纯度SSR鉴定与田间鉴定的一致性研究

Research on SSR molecular marker indentification technique of hybrids purity in cabbage

【目的】分析甘蓝杂交种纯度的SSR分子标记鉴定结果与田间形态学鉴定结果的一致性,寻找一种快速、准确鉴定甘蓝杂交种纯度的方法。【方法】选用SSR分子标记,筛选杂交种具有父母本互补带型的引物,用其鉴定"秦甘50"杂交种的纯度,并与田间常规鉴定结果的一致性进行比较。【结果】300对SSR分子标记引物中,有1对引物SQ1019对"秦甘50"杂交种表现为父母本互补带型;利用引物SQ1019标记鉴定"秦甘50"大田杂交种的纯度为96.3%;"秦甘50"大田杂交种经田间鉴定纯度为96.0%,2种鉴定结果的一致性高达99.7%。【结论】SQ1019是SSR分子标记"秦甘50"杂交种的特异引物,SSR分子标记是一种快速、准确鉴定"秦甘50"杂交种纯度的方法,其结果与田间常规鉴定结果相一致。

[Objective] The research analyzed consistency of SSR molecular marker technique with field morphological observation of hybrids purity in cabbage to seek a quick and accurate identification of seed purity. [Method] We screened out stable codominance banding pattern SSR primers by using SSR molecular marker technique,identified the purity of Qin＇gan 50 hybrids and compared with the result of morphological observation in field. [Result] There were 1 pair of primers SQ1019 which presented stable codomi- nance banding pattern among 300 pairs of screened SSR primers ; The purity of Qin＇gan 50 was 96.3% by SSR molecular marker using SQ1019 ; Field identification with its corresponding plant of SSR was 96.0%. The results indicated that they were the same for hybrid identification between SSR approach and morphological assessment,with the consistency rate of them reaching 99.7%. [Conclusion] SQ1019 is the specific primer to identify seed purity of Qin＇gan 50,the identification between SSR approach and morphological assessment is the same, SSR molecular marker technique is a quick and accurate method to identify seed purity.