人胎肝基质细胞培养上清液中造血生长因子的分析

STUDIES ON HEMOPOIETIC GROWTH FACTORS IN THE SUPERNATANT OF HUMAN FETAL LIVER STROMAL CELLS

采用人胎肝造血基质细胞的体外液体培养技术,结合造血干细胞和祖细胞的体外测试方法,研究了造血基质细胞所释放的造血生长因子与造血干细胞和祖细胞之间的相互作用。结果表明,在适宜的条件下,人胎肝造血基质细胞可在体外传代培养达100d之久。培养过程中,对不同时间收集的培养上清液进行测试的结果表明,这些贴壁细胞可以不断地释放多种造血活性物质。在100d培养过程中,上清液中始终都可以检出CFU-S增殖刺激物活性。培养第24天的上清液中还可检出BPF和GM-CSF活性。这些造血活性物质对CFU-S的生理状态和祖细胞的增殖与分化有着深刻的影响。但是在培养上清液中未检出IL-3样活性物质。

Using the in vitro liquid culture system of human fetal liver stromal cells and the assay methods of hemopoietic stem and progenitor cells, we studied the interactions between stromal cells or the hemopoietic growth factors released by these cells and hemopoietic cells. The results showed that human fetal liver strornal cells could be cultured in the in vitro liquid culture system for a period of 100 days under the optimal conditions with series passages. During the period of culture the supernatant was collected at intervals of various times and was analysed respectively. The fetal liver stromal cells could continuously release several kinds of hemopoietic growth factors into the supernatant in which the CFU-S proliferation stimulator could be found all the time throughout the period of culture. In addition, BPF and GM-CSF could also be found in the supernatant collected on 24th day of culture. These hemopoietic growth factors have profound influences not only on the physiological state of stem cells (CFU-S), but also on the proliferation and differentiation of progenitors. No IL-3 like factors could be found in the supernatant.