反复肺炎婴幼儿血清β-防御素-1和免疫球蛋白A、G、M水平的研究

Serum levels of human β-defensins 1 and immunoglobulins A,G and M in infants with recurrent pneumonia

目的通过检测反复肺炎婴幼儿和健康婴幼儿血清中β-防御素-1(hBD-1)和免疫球蛋白A(IgA)、免疫球蛋白G(IgG)、免疫球蛋白M(IgM)浓度,探讨hBD-1和IgA、IgG、IgM在反复肺炎发病中的可能作用。方法收集35例2～24月龄反复肺炎和35例健康婴幼儿的血清,处理后应用酶联免疫吸附法(ELISA)检测血清中hBD-1浓度,应用免疫比浊法测定血清IgA、IgG、IgM浓度,并分析血清hBD-1与IgA、IgG、IgM之间的相关性。结果反复肺炎组血清hBD-1浓度为14±11μg/mL,显著低于健康对照组(18±11μg/mL),差异有统计学意义(P<0.05)。反复肺炎组血清IgA浓度为1.3±0.6 g/L,显著低于健康对照组(1.5±0.8 g/L),差异有统计学意义(P<0.05)。反复肺炎组血清IgG浓度为9±3 g/L,亦显著低于健康对照组(13±5 g/L),差异有统计学意义(P<0.05)。血清hBD-1与IgA、IgG、IgM之间不存在线性相关关系(P>0.05)。结论反复肺炎婴幼儿存在血清hBD-1、IgA、IgG水平低下的现象,提示呼吸道免疫防御功能存在障碍,这可能是婴幼儿反复肺炎的免疫因素之一;对反复肺炎婴幼儿同时检测hBD-1、IgA、IgG、IgM具有重要的临床意义。

Objective To study the possible role of human β-defensins 1（hBD-1） and immunoglobulins A,G and M（IgA,IgG and IgM） in the development of recurrent pneumonia by measuring serum concentrations of the above indexes in infants with recurrent pneumonia and healthy infants. Methods Serum samples were obtained from 35 healthy children and 35 children aged from 2 to 24 months with recurrent pneumonia.Serum hBD-1 concentration was measured using ELISA.Serum IgA,IgG and IgM concentrations were measured by immunonephelometry.The correlation of hBD-1 with IgA,IgG and IgM was evaluated. Results The serum concentration of hBD-1 in infants with recurrent pneumonia（14±11 μg/mL） was significantly lower than in controls（18±11 μg/mL）（P〈0.05）,as was the serum concentration of IgA in infants with recurrent pneumonia（1.3±0.6 g/L vs 1.5±0.8 g/L;P〈0.05）.The serum concentration of IgG in infants with recurrent pneumonia was also significantly lower than in controls（9±3 g/L vs 13±5 g/L;P〈0.05）.There were no linear relationships between serum hBD-1 and IgA,IgG and IgM（P〉0.05）. Conclusions The serum levels of hBD-1,IgA and IgG decrease in infants with recurrent pneumonia,suggesting disorders in the immune defensive function of the respiratory tract,and this may be one of the immunity related reasons for recurrent pneumonia in infants.It is of great clinical value to measure serum levels of hBD-1,IgA,IgG and IgM in infants with recurrent pneumonia.