miR-29c对脑胶质瘤U251细胞生物学活性的影响

Effect of miR-29c on the biological behavior of human glioma cell U251

目的探讨miR-29c对脑胶质瘤U251细胞生物学活性的影响及可能机制。方法体外培养胶质瘤U251细胞,实验组将miR-29c mimics转染至细胞,miR mimics转染作为对照组。应用实时荧光定量PCR检测转染后miR-29c表达量的改变,MTT实验测定细胞增殖,流式细胞术检测细胞凋亡情况,体外侵袭实验分析miR-29c转染后细胞的体外侵袭能力,Westernblot检测转染后转录因子YY1蛋白的表达。结果荧光定量PCR结果显示:实验组miR-29c表达量为(106.65±15.51),较对照组显著上调(P<0.01)。与对照组比较,实验组U251细胞的增殖能力明显降低(P<0.01),凋亡率增加(P<0.05),体外侵袭能力明显减弱(P<0.01),YY1蛋白表达下调(P<0.01)。结论 miR-29c可能通过调控YY1蛋白的表达抑制胶质瘤细胞的生物活性。

Objective To explore the effect of miR-29c on the biological activity of human glioma cell U251 and its probable mechanism. Methods miR-29c mimics were transfected into human glioma cell U251 cultured in vitro as experimental group and the miR mimics were taken as control group. The expression of miR-29c was measured by real-time fluorescence quantitative PCR （RT-PCR） after transfection. The cell proliferation was determined by MTT assay and cell apoptosis was assayed by flow cytometry （FCM）. The invasive ability of U251 cells＇was observed by Transwell invasion assay before and after transfection with miR-29c mimics. The expression of transcription factor YY1 protein was detected by Western blot. Results RT-PCR results showed that the expression of miR-29c in experimental group was 106.65 ＋ 15.51 after transfection and significantly more than that in control group （P〈0.01）. Compared with control group, the proliferation ability of U251 cells significantly decreased （P〈0.01）, the cell apoptosis rate increased （P〈0.05）, invasive ability in vitro was inhibited （P〈0.01） and the expression of YY1 protein down-regulated in the experimental group （P〈0.01）. Conclusions miR-29c can inhibit the biological behavior of U251 cells, which may be attributed to the regulation of YY1 expression.