CD133两种亚型分子在儿童B系急性淋巴细胞白血病中的表达及其临床意义

Expression of Two Subtype Molecules of CD133 in Childhood with B Linage Acute Lymphoblastic Leukemia and Its Clinical Significance

本研究旨在探讨B系急性淋巴细胞白血病(B-ALL)患儿初诊和治疗33 d时CD133两种亚型分子CD133-1和CD133-2的表达及其与临床预后指标的关系。用流式细胞术检测B-ALL患儿上述两个时间点骨髓单个核细胞的CD133-1、CD133-2表达和治疗33 d时微量残留病(MRD)的变化。结果表明,B-ALL初诊组48例中CD133-1阳性表达18例(37.5%),CD133-2阳性表达30例(62.5%,P<0.05);治疗33 d组25例中CD133-1阳性表达2例(8.0%),CD133-2阳性表达23例(92.0%,P<0.05)。诱导化疗后CD133-1的表达显著减少,但高于正常对照组;而CD133-2的表达下降缓慢。结论:初诊B-ALL患者CD133表达与其性别、年龄、外周血白细胞计数、骨髓幼稚细胞比例、FAB亚型、细胞遗传学异常、融合基因表达、危险等级及完全缓解率等均无相关性。B-ALL患儿CD133-2阳性表达高于CD133-1,CD133表达与CD34表达无相关性。CD133-2的表达与MRD显著相关。

This study was to explore the expression of two subtype molecules of CD133 and its relationship with clinical prognostic factors in childhood with B linage acute lymphoblastic leukemia（B-ALL） at initial diagnosis and the 33rd day of induction chemotherapy.Expression of CD133-1 and CD133-2 in 48 cases of B-ALL and 25 cases at initial diagnosis and the 33rd day of treatment was detected by flow cytometry.Minimal residual disease（MRD） of B-ALL at 33rd day was evaluated by flow cytometry.The results indicated that the expression of CD133-1 was positive in 18 cases（37.5%）,and expression of CD133-2 in 30 cases（62.5%）was positive from 48 cases with newly diagnosed ALL（P〈 0.05）.At 33rd day of treatment,expression of CD133-1 in 2 cases（8.0%） from 25 cases was positive,and expression of CD133-2 in 23 cases（92.0%）was positive（P〈 0.05）.After induction chemotherapy in B-ALL,the expression of CD133-1 decreased significantly,but still higher than that in the normal control group.Compared to expression of CD133-1,expression of CD133-2 decreased slowly.It is concluded that there is no relations among expression of CD133 and sex,age,white blood cell count,percentage of bone marrow blast cells,FAB subtype,cytogenetics,leukemia fusion gene,risk stratification and complete remission rate in childhood B-ALL.The positive expression rates and levels of CD133-2 are higher than those of CD133-1 in B-ALL.There is no statistical correlation between expression of CD133 and CD34 in B-ALL.The expression of CD133-2 is significantly related to the level of MRD.