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基于荧光强度快速判断蛋白可溶性方法的建立

Foundation of Fluorescence Based Protein Solubility Fast Detection Technology
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摘要 目的:建立一种通过观察荧光强度,快速、直观地判断蛋白可溶性的方法。方法:选择抗辐射蛋白CBL502-AA及其突变体CBL502-ΔAA′作为目的蛋白,增强型绿色荧光蛋白作为报告分子,分别构建融合蛋白表达载体;通过SDS-PAGE和荧光观察两种手段检测和比较融合蛋白的可溶性。结果:荧光观察融合蛋白的可溶性与SDS-PAGE检测结果一致。结论:建立了一种基于荧光强度,快速、简单、直观的比较蛋白可溶性的方法。 Objective： To construct a technology detecting protein solubility by observing fluorescence. Methods： We selected radiation protective protein CBL502-AA＇ and it＇s mutation CBL502-AAA＇ as objective proteins, EGFP as reporter protein, constructed EGFP-AA＇ and EGFP-AAA＇ fusion protein expression vector respectively. The protein solubility was compared by parallel SDS-PAGE and fluorescence observing. Results： The protein solubility detected by these two methods was identical. Conclusion： The fluorescence based protein solubility fast detection method is preliminary found.
作者 肖丽霞 叶丙雨 王洋 师明磊 何彰华 张乐之 赵玉军 赵志虎
机构地区 沈阳农业大学畜牧兽医学院 军事医学科学院生物工程研究所
出处 《生物技术通讯》 CAS 2012年第3期415-418,共4页 Letters in Biotechnology
关键词 荧光 蛋白可溶性 绿色荧光蛋白 fluorescence protein solubility green fluorescent protein
分类号 Q503 [生物学—生物化学] Q78 [生物学—分子生物学]
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参考文献10

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生物技术通讯
2012年 第3期

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