百草枯中毒大鼠肺组织中的4-羟基壬烯醛表达和乌司他丁的影响

Expression of the 4-hydroxynone nal in lung tissue in rats with paraquat poisoned and the effect of ulinastatin

目的观察乌司他丁（UTI）对百草枯（PQ）中毒大鼠肺组织中4-羟基壬烯醛（4-HNE）表达的影响。方法将72只SD大鼠按随机数字表法分为对照组、染毒组、UTI干预组，每组24只。染毒组、UTI干预组PQ灌胃（80mg／ks）染毒建立SD大鼠肺损伤模型；对照组组等量生理盐水灌胃。UTI干预组于PQ灌胃后30min腹腔内注射UTI 10万U／kg；对照组、染毒组腹腔注射等量生理盐水。不同处理后12、24、48、72h观察3组大鼠肺组织病理改变及检测肺组织中4HNE表达。结果染毒后12h，染毒组和UTI干预组大鼠肺组织4-HNE表达升高，48h达高峰，72h4-HNE表达下降，但仍较高。与对照组组比较，染毒组和干预组大鼠肺组织4-HNE表达明显升高，差异有统计意义（P〈0．05）。干预组大鼠肺组织4-HNE表达明显低于染毒组，差异有统计意义（P〈0．01）。肺组织病理学观察可见，对照组无明显变化；染毒组、干预组肺泡毛细血管扩张，伴弥漫性肺出血，肺泡腔塌陷，肺泡腔内炎性细胞浸润，其中干预组较染毒组为轻。结论PQ中毒时4-HNE表达增强；UTI能减少4-HNE的产生，减轻PQ中毒大鼠的肺损伤。

Objective To investigate the 4-hydroxynonenal （4-HNE） expression changes and the impact of ulinastatin （UTI）. Methods Seventy-two healthy Sprague-Dawley rats were randomly divided into three groups： the control group, poisoning group and treatment group, with 24 rats in each group. The model of lung injury was established by intragastric PQ （80 mg/kg） administration in poisoning group and treatment group, and 1 mL saline was administered intragastrically in the control group. The rats in treatment group were injected intraperitoneally with UTI （100 000 U/kg） 30 minutes after PQ administration, and the rats in the control group and poisoning group were intraperitoneally injected with the same volume of saline. After different treatments, the pathological changes and the expression of 4-HNE in lung tissue was detected in 12, 24, and 72 h in three groups. Results In the poisoning group and treatment group, the expression of 4-HNE in lung tissue of rats were increased in 12 h after poisoning and reached the peak in 48 h; in 72 h after poisoning, the expression of 4-HNE in lung tissue were decreased, but they were still high. Compared with the control group, the expression of 4-HNE in lung tissue of rats were significantly increased in the poisoning group and treatment group （P〈0.05）. And compared with the poisoning group, the expression of 4-HNE in lung tissue of rats were significantly decreased in the treatment group （P〈0.01）. The pathological changes were observed, irlcluding alveolar capillary expansion, diffuse alveolar hemorrhage and alveolar inflammation cell infiltration, were found in lungs of rats in poisoning group and treatment group. There is no significant change in the control group. Compared with the control group, the expression of 4-HNE in lung tissue significantly increased in poisoning group and treatment group （P〈0.01） , but the expression in treatment group was lower than in poisoning group （P〈0.01）. Conclusion The expression of 4-HNE increased in PQ intoxicated rats. UTI may reduce the expression of 4-HNE and reduce lung injury in PQ intoxicated rats.